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Unfortunately, the demand for the identification of these proteins remains unmet. To overcome this bottleneck, we combined several strategies to identify the proteins detected via MALDI profiling including on-tissue protein extraction using hexafluoroIsopropanol (1,1,1,3,3,3-hexafluoro-2-propanol, HFIP) coupled with two-dimensional cetyl trimethylammonium bromide\/sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis (2D CTAB\/SDS-PAGE) for separation followed by trypsin digestion and MALDI-MS analyses for identification. This strategy was compared with an on-tissue bottom-up strategy that we previously developed. The data reflect the complementarity of the approaches. An increase in the number of specific proteins identified has been established. This approach demonstrates the potential of adapted extraction procedures and the combination of parallel identification approaches for personalized medicine applications. The anatomical context provides important insight into identifying biomarkers and may be considered a first step for tissue-based biomarker research, as well as the extemporaneous examination of biopsies during surgery.<\/jats:p>","DOI":"10.1089\/omi.2013.0176","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T15:21:47Z","timestamp":1400512907000},"page":"374-384","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["HFIP Extraction Followed by 2D CTAB\/SDS-PAGE Separation: A New Methodology for Protein Identification from Tissue Sections after MALDI Mass Spectrometry Profiling for Personalized Medicine Research"],"prefix":"10.1177","volume":"18","author":[{"given":"R\u00e9mi","family":"Longuesp\u00e9e","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Lille 1","place":["France"]},{"name":"Universit\u00e9 de Sherbrooke","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Tastet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille 1","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annie","family":"Desmons","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille 1","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Kerdraon","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille 1","place":["France"]},{"name":"Laboratoire d'Anatomie et de Cytologie Pathologiques","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Day","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Sherbrooke","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Fournier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille 1","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Salzet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille 1","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,6]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.08.032"},{"key":"e_1_3_3_3_1","first-page":"998","article-title":"Expression of carbonic anhydrase I or II and correlation to clinical aspects of colorectal cancer","volume":"47","author":"Bekku S","year":"2000","unstructured":"Bekku S, , Mochizuki H, , Yamamoto T, , Ueno H, , Takayama E, , and Tadakuma T. 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