{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:54:31Z","timestamp":1777928071157,"version":"3.51.4"},"reference-count":48,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Infectious diseases and increasing antibiotic resistance among diverse classes of microbes are global health concerns and a prime focus of omics systems science applications in novel drug discovery. Plumbagin is a plant-derived naphthoquinone, a natural product that exhibits antibacterial activity against gram-positive bacteria. In the present study, we investigated the antimicrobial effects of plumbagin against\n                    <jats:italic toggle=\"yes\">Bacillus subtilis<\/jats:italic>\n                    using two complementary proteomics techniques: two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantification (iTRAQ). Comparative quantitative proteomics analysis of plumbagin treated and untreated control samples identified differential expression of 230 proteins (1% FDR, 1.5 fold-change and \u22652 peptides) in\n                    <jats:italic toggle=\"yes\">B. subtilis<\/jats:italic>\n                    after plumbagin treatment. Pathway analysis involving the differentially expressed proteins suggested that plumbagin effectively increases heme and protein biosynthesis, whereas fatty acid synthesis was significantly reduced. Gene expression and metabolic activity assays further corroborated the proteomics findings. We anticipate that plumbagin blocks the cell division by altering the membrane permeability required for energy generation. This is the first report, to the best of our knowledge, offering new insights, at proteome level, for the putative mode(s) of action of plumbagin and attendant cellular targets in\n                    <jats:italic toggle=\"yes\">B. subtilis<\/jats:italic>\n                    . The findings also suggest new ways forward for the modern omics-guided drug target discovery, building on traditional plant medicine.\n                  <\/jats:p>","DOI":"10.1089\/omi.2014.0099","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T16:03:50Z","timestamp":1420560230000},"page":"12-23","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Proteomics Analyses of\n                    <i>Bacillus subtilis<\/i>\n                    after Treatment with Plumbagin, a Plant-Derived Naphthoquinone"],"prefix":"10.1177","volume":"19","author":[{"given":"Panga Jaipal","family":"Reddy","sequence":"first","affiliation":[{"name":"Indian Institute of Technology Bombay","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandipan","family":"Ray","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Bombay","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gajanan J.","family":"Sathe","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Manipal University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.S. Keshava","family":"Prasad","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srikanth","family":"Rapole","sequence":"additional","affiliation":[{"name":"Proteomics Lab","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dulal","family":"Panda","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Bombay","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeeva","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Bombay","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi800730q"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12429"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01317-10"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.2.456-463.2006"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2012.02.022"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.3402\/ehtj.v6i0.20632"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.08.019"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.11.3072-3080.2000"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4028(200110)41:5<253::AID-JOBM253>3.0.CO;2-R"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762003000700017"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(97)00125-7"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02356.x"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2MD20127E"},{"key":"e_1_3_3_15_1","first-page":"785","article-title":"Anticancer mechanism of plumbagin, a natural compound, on non-small cell lung cancer cells","volume":"28","author":"Gomathinayagam R","year":"2008","unstructured":"Gomathinayagam R, , Sowmyalakshmi S, , Mardhatillah F, , Kumar R, , Akbarsha MA, , and Damodaran C. (2008). Anticancer mechanism of plumbagin, a natural compound, on non-small cell lung cancer cells. Anticancer Res, 28, 785\u2013792.","journal-title":"Anticancer Res"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01606-07"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06173.x"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0250"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001413"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20634"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90581-G"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi602573e"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2013.0100"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03875.x"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.23.6928-6937.2003"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.11.3259-3265.2000"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.2001.4674"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-17-84"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.110.170852"},{"key":"e_1_3_3_32_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-1-97"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.11.3392-3401.1999"},{"key":"e_1_3_3_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000956107"},{"key":"e_1_3_3_35_1","first-page":"615","article-title":"Chemical modification of the lactose carrier of Escherichia coli by plumbagin, phenylarsinoxide or diethylpyrocarbonate affects the binding of galactoside","volume":"137","author":"Neuhaus JM","year":"1983","unstructured":"Neuhaus JM, , and Wright JK. (1983). Chemical modification of the lactose carrier of Escherichia coli by plumbagin, phenylarsinoxide or diethylpyrocarbonate affects the binding of galactoside. Eur J, 137, 615\u2013621.","journal-title":"Eur J"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00602-07"},{"key":"e_1_3_3_37_1","first-page":"284","article-title":"Integrating \u201comics\u201d technologies to conceptualize dynamic antimicrobial peptide responses","volume":"3","author":"Plichta JK","year":"2012","unstructured":"Plichta JK, , Nienhouse V, , and Radek KA. (2012). Integrating \u201comics\u201d technologies to conceptualize dynamic antimicrobial peptide responses. Front Immunol 2012, 3, 284.","journal-title":"Front Immunol 2012"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00011-12"},{"key":"e_1_3_3_39_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070891"},{"key":"e_1_3_3_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.08.013"},{"key":"e_1_3_3_41_1","doi-asserted-by":"publisher","DOI":"10.2174\/15701646113106660004"},{"key":"e_1_3_3_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.5"},{"key":"e_1_3_3_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.468"},{"key":"e_1_3_3_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005485107"},{"key":"e_1_3_3_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097514"},{"key":"e_1_3_3_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr373"},{"key":"e_1_3_3_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01380-12"},{"key":"e_1_3_3_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr483"},{"key":"e_1_3_3_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.16.4458-4465.2000"}],"container-title":["OMICS: A Journal of Integrative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/omi.2014.0099","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/omi.2014.0099","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/omi.2014.0099","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:59:09Z","timestamp":1777676349000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/omi.2014.0099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1089\/omi.2014.0099"],"URL":"https:\/\/doi.org\/10.1089\/omi.2014.0099","relation":{},"ISSN":["1536-2310","1557-8100"],"issn-type":[{"value":"1536-2310","type":"print"},{"value":"1557-8100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1]]}}}