{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:32:01Z","timestamp":1790818321177,"version":"4.1.0"},"reference-count":73,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic toggle=\"yes\">Leishmania donovani<\/jats:italic>\n                    is a kinetoplastid protozoan that causes a severe and fatal disease\n                    <jats:italic toggle=\"yes\">kala-azar,<\/jats:italic>\n                    or visceral leishmaniasis.\n                    <jats:italic toggle=\"yes\">L. donovani<\/jats:italic>\n                    infects human host after the phlebotomine sandfly takes a blood meal and resides within the phagolysosome of infected macrophages. Previous studies on host\u2013parasite interactions have not focused on\n                    <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                    organelles and the role that they play in the survival of this parasite within macrophages.\n                    <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                    possess glycosomes that are unique and specialized subcellular microbody organelles. Glycosomes are known to harbor most peroxisomal enzymes and, in addition, they also possess nine glycolytic enzymes. In the present study, we have carried out proteomic profiling using high resolution mass spectrometry of a sucrose density gradient-enriched glycosomal fraction isolated from\n                    <jats:italic toggle=\"yes\">L. donovani<\/jats:italic>\n                    promastigotes. This study resulted in the identification of 4022 unique peptides, leading to the identification of 1355 unique proteins from a preparation enriched in\n                    <jats:italic toggle=\"yes\">L. donovani<\/jats:italic>\n                    glycosomes. Based on protein annotation, 566 (41.8%) were identified as hypothetical proteins with no known function. A majority of the identified proteins are involved in metabolic processes such as carbohydrate, lipid, and nucleic acid metabolism. Our present proteomic analysis is the most comprehensive study to date to map the proteome of\n                    <jats:italic toggle=\"yes\">L. donovani<\/jats:italic>\n                    glycosomes.\n                  <\/jats:p>","DOI":"10.1089\/omi.2014.0163","type":"journal-article","created":{"date-parts":[[2015,3,6]],"date-time":"2015-03-06T16:04:19Z","timestamp":1425657859000},"page":"157-170","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":39,"title":["Comprehensive Proteomics Analysis of Glycosomes from\n                    <i>Leishmania donovani<\/i>"],"prefix":"10.1177","volume":"19","author":[{"given":"Mahendra D.","family":"Jamdhade","sequence":"first","affiliation":[{"name":"National Centre for Cell Science","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsh","family":"Pawar","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Rajiv Gandhi University for Health Sciences","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandip","family":"Chavan","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Manipal University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gajanan","family":"Sathe","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Manipal University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.K.","family":"Umasankar","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiran N.","family":"Mahale","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanwi","family":"Dixit","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil K.","family":"Madugundu","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Pondicherry University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.S. 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