{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T16:26:53Z","timestamp":1783441613150,"version":"3.54.6"},"reference-count":48,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Global health therapeutics is a rapidly emerging facet of postgenomics medicine. In this connection,\n                    <jats:italic toggle=\"yes\">Brucella melitensis<\/jats:italic>\n                    is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. Presently, no licensed vaccines are available for human brucellosis. Here, we report the identification of potential vaccine candidates against\n                    <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic>\n                    using a reverse vaccinology approach. Based on a systematic screening of exoproteome and secretome of\n                    <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic>\n                    16M, we identified eight proteins as potential vaccine candidates, including LPS-assembly protein LptD, a polysaccharide export protein, a cell surface protein, heme transporter BhuA, flagellin FliC, 7-alpha-hydroxysteroid dehydrogenase, immunoglobulin-binding protein EIBE, and hemagglutinin. Among these, the roles of BhuA and hemagglutinin in the virulence of\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    are essential to establish infection. Roles of other proteins in the virulence are yet to be studied. Prediction of protein\u2013protein interactions revealed that these proteins can interact with other proteins involved in virulence, secretion system, metabolism, and transport. From these eight potential vaccine candidates, we predicted three surface exposed novel antigenic epitopes that can induce both B-cell and T-cell immune responses. These peptides can be used for the development of either exclusive peptide vaccines or multi-component vaccines against human brucellosis. Reverse vaccinology is an important strategy for discovery of novel global health therapeutics.\n                  <\/jats:p>","DOI":"10.1089\/omi.2015.0105","type":"journal-article","created":{"date-parts":[[2015,10,19]],"date-time":"2015-10-19T15:06:16Z","timestamp":1445267176000},"page":"722-729","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Novel Vaccine Candidates against\n                    <i>Brucella melitensis<\/i>\n                    Identified through Reverse Vaccinology Approach"],"prefix":"10.1177","volume":"19","author":[{"given":"Udayakumar S.","family":"Vishnu","sequence":"first","affiliation":[{"name":"Madurai Kamaraj University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagadesan","family":"Sankarasubramanian","sequence":"additional","affiliation":[{"name":"Madurai Kamaraj University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paramasamy","family":"Gunasekaran","sequence":"additional","affiliation":[{"name":"Madurai Kamaraj University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeyaprakash","family":"Rajendhran","sequence":"additional","affiliation":[{"name":"Madurai Kamaraj 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