{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:53:37Z","timestamp":1777928017508,"version":"3.51.4"},"reference-count":47,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Enteritidis causes food-borne gastroenteritis by the two type three secretion systems (TTSS). TTSS-1 mediates invasion through intestinal lining, and TTSS-2 facilitates phagocytic survival. The pathogens' ability to infect effectively under TTSS-1-deficient background in host's phagocytes is poorly understood. Therefore, pathobiological understanding of TTSS-1-defective nontyphoidal Salmonellosis is highly important. We performed a comparative global proteomic analysis of the isogenic TTSS-1 mutant of\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Enteritidis (M1511) and its wild-type isolate P125109. Our results showed 43 proteins were differentially expressed. Functional annotation further revealed that differentially expressed proteins belong to pathogenesis, tRNA and ncRNA metabolic processes. Three proteins, tryptophan subunit alpha chain, citrate lyase subunit alpha, and hypothetical protein 3202, were selected for\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    analysis based on their functional annotations. Deletion mutants generated for the above proteins in the M1511 strain showed reduced intracellular survival inside macrophages\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . In sum, this study provides mass spectrometry-based evidence for seven hypothetical proteins, which will be subject of future investigations. Our study identifies proteins influencing virulence of\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    in the host. The study complements and further strengthens previously published research on proteins involved in enteropathogenesis of\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    and extends their role in noninvasive Salmonellosis.\n                  <\/jats:p>","DOI":"10.1089\/omi.2017.0112","type":"journal-article","created":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T13:58:50Z","timestamp":1508421530000},"page":"571-583","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["\u201cOmics\u201d of Food-Borne Gastroenteritis: Global Proteomic and Mutagenic Analysis of\n                    <i>Salmonella enterica<\/i>\n                    Serovar Enteritidis"],"prefix":"10.1177","volume":"21","author":[{"given":"Aryashree","family":"Arunima","sequence":"first","affiliation":[{"name":"KIIT University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soujanya D.","family":"Yelamanchi","sequence":"additional","affiliation":[{"name":"KIIT University","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandrashekhar","family":"Padhi","sequence":"additional","affiliation":[{"name":"KIIT University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangeeta","family":"Jaiswal","sequence":"additional","affiliation":[{"name":"KIIT University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Ryan","sequence":"additional","affiliation":[{"name":"KIIT University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhawna","family":"Gupta","sequence":"additional","affiliation":[{"name":"KIIT University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gajanan","family":"Sathe","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayshree","family":"Advani","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harsha","family":"Gowda","sequence":"additional","affiliation":[{"name":"KIIT University","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.S. 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