{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:28:34Z","timestamp":1789964914229,"version":"4.0.1"},"reference-count":63,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Male infertility is an important global health burden that can benefit from novel biomarkers and diagnostics innovation. Aberrant methylation of the imprinted genes\n                    <jats:italic toggle=\"yes\">H19<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">SNRPN<\/jats:italic>\n                    (small nuclear ribonucleoprotein polypeptide N) in sperm DNA has been implicated in abnormal sperm parameters and male infertility. However, whether certain methylation patterns of one or multiple CpG sites within an imprinted gene are pathological for multiple sperm defects remains poorly understood. To examine the diagnostic potential of certain methylation patterns of CpG sites for multiphenotype defects in human sperm, the sperm DNA methylation patterns of individual CpG sites within imprinting control regions (ICRs) of imprinted genes\n                    <jats:italic toggle=\"yes\">H19<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">SNRPN<\/jats:italic>\n                    were measured by bisulfite pyrosequencing in a Han Chinese population sample: 39 oligoasthenozoospermia (OA) patients, 36 asthenoteratozoospermia (AT) patients, and 50 normozoospermia (N) controls. A partial least squares discriminant analysis model was built with the CpG sites as independent variables. Among the 16 CpG sites screened, the methylation patterns of eight CpG sites within\n                    <jats:italic toggle=\"yes\">H19<\/jats:italic>\n                    -ICR (CpG sites 1, 6\u20139, 12 and 15\u201316), and eight CpG sites within\n                    <jats:italic toggle=\"yes\">SNRPN<\/jats:italic>\n                    -ICR (CpG sites 2, 5\u20136, 8\u201310, 13, and 16) correctly classified 74.4% and 72.0% of the samples in terms of male fertile status, respectively. Furthermore, by combination of these 16 selected CpG sites within ICRs of\n                    <jats:italic toggle=\"yes\">H19<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">SNRPN<\/jats:italic>\n                    , 88.0% of the samples could be successfully classified. Our study demonstrates that methylation profiles of CpG sites within ICRs of imprinted genes\n                    <jats:italic toggle=\"yes\">H19<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">SNRPN<\/jats:italic>\n                    may potentially serve as epigenomic biomarkers for assessment of infertility in men with multiple sperm defects. Further studies in independent population samples are called for diagnostic significance of methylation patterns of CpG sites within imprinted genes.\n                  <\/jats:p>","DOI":"10.1089\/omi.2018.0019","type":"journal-article","created":{"date-parts":[[2018,4,30]],"date-time":"2018-04-30T14:26:16Z","timestamp":1525098376000},"page":"354-364","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Novel Epigenomic Biomarkers of Male Infertility Identified by Methylation Patterns of CpG Sites Within Imprinting Control Regions of\n                    <i>H19<\/i>\n                    and\n                    <i>SNRPN<\/i>\n                    Genes"],"prefix":"10.1177","volume":"22","author":[{"given":"Hongli","family":"Peng","sequence":"first","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panlin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Air Force General Hospital","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaonan","family":"Liu","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manshu","family":"Song","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]},{"name":"Edith Cowan University","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Capital Medical University","place":["China"]},{"name":"Municipal Key Laboratory of Clinical Epidemiology","place":["China"]},{"name":"Edith Cowan University","place":["Australia"]},{"name":"Taishan Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,5]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.03.022"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2010.63"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2008.061820"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19960401)23:4<263::AID-CYTO1>3.0.CO;2-I"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2009.117"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2013.10.010"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.1.28"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02836.x"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/367926"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.21743"},{"key":"e_1_3_3_12_1","first-page":"771","article-title":"Disorders of spermatogenesis in people working at the clean-up of the Chernobyl nuclear power plant accident","volume":"33","author":"Cheburakov YY","year":"1993","unstructured":"Cheburakov YY, , Cheburakova OP. 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