{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T01:19:27Z","timestamp":1789262367392,"version":"build-2803163510"},"reference-count":70,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Next-generation sequencing approaches have revolutionized genomic medicine and enabled rapid diagnosis for several diseases. These approaches are widely used for pathogen detection in several infectious diseases. Lyme disease is a tick-borne infectious disease, which affects multiple organs. The causative organism is a spirochete,\n                    <jats:italic toggle=\"yes\">Borrelia burgdorferi<\/jats:italic>\n                    , which is transmitted by ticks. Lyme disease can be treated easily if detected early, but its diagnosis is often delayed or is incorrect leading to a chronic debilitating condition. Current confirmatory diagnostic tests for Lyme disease rely on detection of antigens derived from\n                    <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                    , which are prone to both false positives and false negatives. Instead of focusing only on the human host for the diagnosis of Lyme disease, one could also attempt to identify the vector (tick) and the causative organism carried by the tick. Since all ticks do not transmit Lyme disease, it can be informative to accurately identify the tick from the site of bite, which is often observed by the patient and discarded. However, identifying ticks based on morphology alone requires a trained operator and can still be incorrect. Thus, we decided to take a molecular approach by sequencing DNA and RNA from a tick collected from an individual bitten by the tick. Using next-generation sequencing, we confirmed the identity of the tick as a dog tick,\n                    <jats:italic toggle=\"yes\">Dermacentor variabilis<\/jats:italic>\n                    , and did not identify any pathogenic bacterial sequences, including\n                    <jats:italic toggle=\"yes\">Borrelia<\/jats:italic>\n                    species. Despite the limited availability of nucleotide sequences for many types of ticks, our approach correctly identified the tick species. This proof-of-principle study demonstrates the potential of next-generation sequencing in the diagnosis of tick-borne infections, which can also be extended to other zoonotic diseases.\n                  <\/jats:p>","DOI":"10.1089\/omi.2018.0089","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T10:51:14Z","timestamp":1534243874000},"page":"565-574","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["A Next-Generation Sequencing-Based Molecular Approach to Characterize a Tick Vector in Lyme Disease"],"prefix":"10.1177","volume":"22","author":[{"given":"Anil K.","family":"Madugundu","sequence":"first","affiliation":[{"name":"Manipal Academy of Higher Education (MAHE)","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Johns Hopkins University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babylakshmi","family":"Muthusamy","sequence":"additional","affiliation":[{"name":"Manipal Academy of Higher Education (MAHE)","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreelakshmi K.","family":"Sreenivasamurthy","sequence":"additional","affiliation":[{"name":"Manipal Academy of Higher Education (MAHE)","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Johns Hopkins University School of Medicine"},{"name":"National Institute of Mental Health and Neurosciences","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra","family":"Bhavani","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Manipal Academy of Higher Education (MAHE)","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bankatesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health and Neurosciences","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna R.","family":"Murthy","sequence":"additional","affiliation":[{"name":"Manipal Academy of Higher Education (MAHE)","place":["India"]},{"name":"Institute of Bioinformatics","place":["India"]},{"name":"Vittala International Institute of Ophthalmology","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raju","family":"Ravikumar","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health and Neurosciences","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhilesh","family":"Pandey","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine"},{"name":"Johns Hopkins University School of Medicine"},{"name":"Johns Hopkins University School of Medicine"},{"name":"Johns Hopkins University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,8]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6453a1"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2007.01160.x"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1008.030882"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.47.249"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-79"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/09273940903359733"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365549309169672"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v3.i5.430"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2015.05.014"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-004-0139-x"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2008.03914.x"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3164-3168.2004"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1981-7"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2012.07.003"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-010-9340-5"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2008.03.006"},{"key":"e_1_3_3_18_1","first-page":"680","article-title":"Lyme disease and related tick-borne illnesses","volume":"33","author":"Edlow JA","year":"1999","unstructured":"Edlow JA. 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