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In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant\n                    <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                    clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the strain C2620 indicated the presence of antibiotic resistance genes characteristic of the\n                    <jats:italic toggle=\"yes\">vanB<\/jats:italic>\n                    cluster. To further investigate the transcriptome of this pathogenic strain, we used whole genome sequencing and RNA-sequencing to detect and quantify the genes expressed. In parallel, we used two-dimensional gel electrophoresis followed by MALDI-TOF\/MS (Matrix-assisted laser desorption\/ionization-Time-of-flight\/Mass spectrometry) to identify the proteins in the proteome. We studied the membrane and cytoplasm subproteomes separately. From a total of 207 analysis spots, we identified 118 proteins. The protein list was compared to the results obtained from the full transcriptome assay. Several genes and proteins related to stress and cellular response were identified, as well as some linked to antibiotic and drug responses, which is consistent with the known state of multiresistance. Even though the correlation between transcriptome and proteome data is not yet fully understood, the use of multiomics approaches has proven to be increasingly relevant to achieve deeper insights into the survival ability of pathogenic bacteria found in health care facilities.\n                  <\/jats:p>","DOI":"10.1089\/omi.2019.0164","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T14:06:32Z","timestamp":1582121192000},"page":"81-95","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Multiomics Substrates of Resistance to Emerging Pathogens? Transcriptome and Proteome Profile of a Vancomycin-Resistant\n                    <i>Enterococcus faecalis<\/i>\n                    Clinical Strain"],"prefix":"10.1177","volume":"24","author":[{"given":"Lu\u00eds","family":"Pinto","sequence":"first","affiliation":[{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro, Vila Real, Portugal."},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro, Vila Real, Portugal."},{"name":"Veterinary Science Department, University of Tr\u00e1s-os-Montes and Alto Douro, Vila Real, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[{"name":"\u00c1rea de Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concha","family":"Gil","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo M.","family":"Santos","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Faculty of Science and Technology, Nova University of Lisbon, Lisbon, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Lu\u00eds","family":"Capelo","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Faculty of Science and Technology, Nova University of Lisbon, Lisbon, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edtor","family":"Borges","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health, Lisbon, 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