{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:55:24Z","timestamp":1777928124184,"version":"3.51.4"},"reference-count":55,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:p>\n                    Bacterial vaginosis (BV) is highly common, adversely affecting the health of millions of women. New therapeutic targets and diagnostics are urgently needed for BV. Microbiome research offers new prospects in this context. We report here original findings on changes in the vaginal microbiome in pregnant and nonpregnant women with BV. Reproductive age women were recruited for this study after a clinical examination. The total sample (\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    \u2009=\u200933) included four study groups: (1) healthy nonpregnant women (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u20099), (2) nonpregnant women with symptomatic BV (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200911), (3) healthy pregnant women without BV (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u20096), and (4) pregnant women with symptomatic BV (\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    \u2009=\u20097). The vaginal microbiota in healthy women was less diverse, with dominance of a single genus,\n                    <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                    . Six major phyla appeared upon taxonomic analysis of the bacterial sequences: Firmicutes, Actinobacteria, Proteobacteria, Tenericutes, Bacteroidetes, and Fusobacteria. For instance, Firmicutes had a significantly higher abundance (98.3%) in the nonpregnant healthy group and 94.3% in pregnant healthy group, compared with nonpregnant (49.7%) and pregnant (67%) women with BV (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.003). Moreover, women with BV had significant increases in representation of Actinobacteria, Fusobacteria, and Bacteroidete\n                    <jats:italic toggle=\"yes\">s<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.0003, 0.004, and 0.01, respectively). Although the\n                    <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                    genus was predominant in healthy women,\n                    <jats:italic toggle=\"yes\">Gardnerella<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Atopobium<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Sneathia<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Prevotella<\/jats:italic>\n                    significantly increased in nonpregnant women with BV (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.001, 0.014, 0.004, and 0.012, respectively). Dysbiosis of\n                    <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                    in pregnant women with BV was accompanied by increased prevalence of the\n                    <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                    genus. These findings contribute new insights toward microbiome diagnostics and therapeutics innovation in BV.\n                  <\/jats:p>","DOI":"10.1089\/omi.2020.0096","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T14:11:56Z","timestamp":1600697516000},"page":"602-614","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Changes in Vaginal Microbiome in Pregnant and Nonpregnant Women with Bacterial Vaginosis: Toward Microbiome Diagnostics?"],"prefix":"10.1177","volume":"24","author":[{"given":"Ibrahim","family":"Mohamed","sequence":"first","affiliation":[{"name":"Quality Control Department, Medical Union Pharmaceuticals Co. Main Factory, Ismailia, Egypt."},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Zakeer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marwa","family":"Azab","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amro","family":"Hanora","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,10,5]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph110706979"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2019.0144"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giy054"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(83)91112-9"},{"key":"e_1_3_5_6_1","first-page":"32","article-title":"A new method for non-parametric multivariate analysis of variance","volume":"26","author":"Anderson MJ","year":"2001","unstructured":"Anderson MJ. 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