{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T14:21:56Z","timestamp":1782224516555,"version":"3.54.5"},"reference-count":66,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>\n                    Cigarette smoking is the major cause of chronic inflammatory diseases such as chronic obstructive pulmonary disease (COPD). It is paramount to develop pharmacological interventions and delivery strategies against the cigarette smoke (CS) associated oxidative stress in COPD. This study in Wistar rats examined cysteamine in nanoemulsions to counteract the CS distressed microenvironment.\n                    <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                    , 28 days of CS and 15 days of cysteamine nanoemulsions treatment starting on 29th day consisting of oral and inhalation routes were established in Wistar rats. In addition, we conducted inflammatory and epithelial-to-mesenchymal transition (EMT) studies\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    in human bronchial epithelial cell lines (BEAS2B) using 5% CS extract. Inflammatory and anti-inflammatory markers, such as tumor necrosis factor-alpha (TNF-\u03b1), interleukin (IL)-6, IL-1\u03b2, IL-8, IL-10, and IL-13, have been quantified in bronchoalveolar lavage fluid (BALF) to evaluate the effects of the cysteamine nanoemulsions in normalizing the diseased condition. Histopathological analysis of the alveoli and the trachea showed the distorted, lung parenchyma and ciliated epithelial barrier, respectively. To obtain mechanistic insights into the CS COPD rat model, \u201cshotgun\u201d proteomics of the lung tissues have been carried out using high-resolution mass spectrometry wherein genes such as\n                    <jats:italic toggle=\"yes\">ABI1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">PPP3CA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">PSMA2<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">FBLN5<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">ACTG1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">CSNK2A1<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ECM1<\/jats:italic>\n                    exhibited significant differences across all the groups. Pathway analysis showed autophagy, signaling by receptor tyrosine kinase, cytokine signaling in immune system, extracellular matrix organization, and hemostasis, as the major contributing pathways across all the studied groups. This work offers new preclinical findings on how cysteamine taken orally or inhaled can combat CS-induced oxidative stress.\n                  <\/jats:p>","DOI":"10.1089\/omi.2023.0074","type":"journal-article","created":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T09:52:11Z","timestamp":1692093131000},"page":"338-360","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["A Proteomics Investigation of Cigarette Smoke Exposed Wistar Rats Revealed Improved Anti-Inflammatory Effects of the Cysteamine Nanoemulsions Delivered\n                    <i>via<\/i>\n                    Inhalation"],"prefix":"10.1177","volume":"27","author":[{"given":"Gautam","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati","family":"Pund","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."},{"name":"Biobay, Ahmedabad, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajkumar","family":"Govindan","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."},{"name":"Department of Biomedical Engineering, Hajim School of Engineering &amp; Applied Sciences, University of Rochester, Rochester, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehar Un","family":"Nissa","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deeptarup","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanniya","family":"Middha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Panjab University, Chandigarh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koustav","family":"Ganguly","sequence":"additional","affiliation":[{"name":"Unit of Integrative Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh Padukudru","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, JSS Medical College, JSSAHER, Mysore, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinti","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5159-6834","authenticated-orcid":false,"given":"Sanjeeva","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,8,25]]},"reference":[{"key":"e_1_3_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2020.119721"},{"key":"e_1_3_6_3_1","article-title":"A Protein Free Surfactant Composition for Pulmonary Diseases and a Process for Preparing the Same","author":"Banerjee R","year":"2013","unstructured":"Banerjee R, , Kaviratna A. A Protein Free Surfactant Composition for Pulmonary Diseases and a Process for Preparing the Same. United States Pat Appl Publ, 2013; US20130323318A1. Available from: https:\/\/patents.google.com\/patent\/US20130323318","journal-title":"United States Pat Appl Publ"},{"key":"e_1_3_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.04.015"},{"key":"e_1_3_6_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0TB00158A"},{"key":"e_1_3_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1675"},{"key":"e_1_3_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.1c00511"},{"key":"e_1_3_6_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6842"},{"key":"e_1_3_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.11.023"},{"key":"e_1_3_6_10_1","first-page":"2732017","article-title":"Inhaled therapy in respiratory disease: The complex interplay of pulmonary kinetic processes","volume":"2018","author":"Borghardt JM","year":"2018","unstructured":"Borghardt JM, , Kloft C, , Sharma A. Inhaled therapy in respiratory disease: The complex interplay of pulmonary kinetic processes. Can Respir J, 2018; 2018:2732017; doi: 10.1155\/2018\/2732017","journal-title":"Can Respir J"},{"key":"e_1_3_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60988-4"},{"key":"e_1_3_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46732-5"},{"key":"e_1_3_6_13_1","article-title":"Association between high density lipoproteins (HDL) cholesterol and CT percent emphysema","volume":"2878","author":"Burkart KM","year":"2010","unstructured":"Burkart KM, , Ahmed FS, , Watson K, et al. Association between high density lipoproteins (HDL) cholesterol and CT percent emphysema. The MESA lung study. Am Thorac Soc Int Conf Meet Abstr, 2010; A2878; doi: 10.1164\/AJRCCM-CONFERENCE.2010.181.1_MEETINGABSTRACTS.A2878","journal-title":"The MESA lung study. Am Thorac Soc Int Conf Meet Abstr"},{"key":"e_1_3_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr200345y"},{"issue":"63","key":"e_1_3_6_15_1","first-page":"OA1604","article-title":"Overexpression of LincR-PICALM promotes lung cancer proliferation and metastasis by MiR-199a-3p suppression and cis-regulation","volume":"54","author":"Chen Z","year":"2019","unstructured":"Chen Z, , Huang M, , Ji N, et al. Overexpression of LincR-PICALM promotes lung cancer proliferation and metastasis by MiR-199a-3p suppression and cis-regulation. Eur Respir J, 2019; 54(Suppl 63):OA1604; doi: 10.1183\/13993003.CONGRESS-2019.OA1604","journal-title":"Eur Respir J"},{"key":"e_1_3_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.116"},{"key":"e_1_3_6_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-783X-15-S2-27"},{"key":"e_1_3_6_18_1","first-page":"140","article-title":"Cytoplasmic Preassembly and Trafficking of Axonemal Dyneins","author":"Desai PB","year":"2018","unstructured":"Desai PB, , Dean AB, , Mitchell DR. Cytoplasmic Preassembly and Trafficking of Axonemal Dyneins. In: Dyneins: Strucuture, Biology and Disease (Second Edition. 2018; pp. 140\u2013161; doi: 10.1016\/B978-0-12-809471-6.00004-8","journal-title":"In: Dyneins: Strucuture, Biology and Disease (Second Edition"},{"key":"e_1_3_6_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.49.8.825"},{"key":"e_1_3_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91742-x"},{"key":"e_1_3_6_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.548243"},{"key":"e_1_3_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR120.002155"},{"key":"e_1_3_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2013.02.006"},{"key":"e_1_3_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imu.2020.100446"},{"key":"e_1_3_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.01.002"},{"key":"e_1_3_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2014.01.003"},{"key":"e_1_3_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep07085"},{"key":"e_1_3_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2009.01.001"},{"key":"e_1_3_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_3_6_30_1","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00658-2020"},{"key":"e_1_3_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2015.09.012"},{"key":"e_1_3_6_32_1","first-page":"683","article-title":"Pharmacokinetics, bioavailability, excretion and metabolism studies of akebia saponin D in rats: Causes of the ultra-low oral bioavailability and metabolic pathway","volume":"12","author":"Li P","year":"2021","unstructured":"Li P, , Peng J, , Li Y, et al. Pharmacokinetics, bioavailability, excretion and metabolism studies of akebia saponin D in rats: Causes of the ultra-low oral bioavailability and metabolic pathway. Front Pharmacol, 2021; 12:683; doi: 10.3389\/FPHAR.2021.621003\/BIBTEX","journal-title":"Front Pharmacol"},{"key":"e_1_3_6_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000008556"},{"key":"e_1_3_6_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302584"},{"key":"e_1_3_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2018.01.002"},{"key":"e_1_3_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(01)00105-3"},{"key":"e_1_3_6_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01600"},{"key":"e_1_3_6_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-125"},{"key":"e_1_3_6_39_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-0105.177703"},{"key":"e_1_3_6_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.07.060178.002251"},{"key":"e_1_3_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"},{"key":"e_1_3_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.08.011"},{"key":"e_1_3_6_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.1c00491"},{"key":"e_1_3_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00676.x"},{"key":"e_1_3_6_45_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050541"},{"key":"e_1_3_6_46_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4874"},{"key":"e_1_3_6_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-015-0225-3"},{"key":"e_1_3_6_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.9b00697"},{"issue":"10","key":"e_1_3_6_49_1","first-page":"356","article-title":"Effects of cigarette smoking on histology of trachea and lungs of albino rat","volume":"3","author":"Shraideh Z","year":"2013","unstructured":"Shraideh Z, , Al-Awaida WJ, , Badran DH. Effects of cigarette smoking on histology of trachea and lungs of albino rat. Res Opin Anim Vet Sci, 2013; 3(10):356\u2013362.","journal-title":"Res Opin Anim Vet Sci"},{"key":"e_1_3_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2017.10.018"},{"key":"e_1_3_6_51_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0297OC"},{"key":"e_1_3_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.152667"},{"key":"e_1_3_6_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph15051033"},{"key":"e_1_3_6_54_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.18078"},{"key":"e_1_3_6_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_3_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2021.105812"},{"key":"e_1_3_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2008.08.001"},{"key":"e_1_3_6_58_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20170880"},{"key":"e_1_3_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2013.08.013"},{"key":"e_1_3_6_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-011-9360-x"},{"key":"e_1_3_6_61_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.96.09010024"},{"key":"e_1_3_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2018.12.013"},{"key":"e_1_3_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2007.07.012"},{"issue":"1","key":"e_1_3_6_64_1","first-page":"13","article-title":"The significance of serum interleukin-8 in acute exacerbations of chronic obstructive pulmonary disease","volume":"17","author":"Zhang J","year":"2018","unstructured":"Zhang J, , Bai C. The significance of serum interleukin-8 in acute exacerbations of chronic obstructive pulmonary disease. Tanaffos, 2018; 17(1):13.","journal-title":"Tanaffos"},{"key":"e_1_3_6_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12282-021-01221-4"},{"issue":"4","key":"e_1_3_6_66_1","first-page":"953","article-title":"The relationship between vitamin E plasma and BAL concentrations, SOD activity and ventilatory support measures in critically ill patients","volume":"10","author":"Ziaie S","year":"2011","unstructured":"Ziaie S, , Jamaati H, , Hajimahmoodi M, et al. The relationship between vitamin E plasma and BAL concentrations, SOD activity and ventilatory support measures in critically ill patients. Iran J Pharm Res, 2011; 10(4):953.","journal-title":"Iran J Pharm Res"},{"key":"e_1_3_6_67_1","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S131877"}],"container-title":["OMICS: A Journal of Integrative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/omi.2023.0074","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/omi.2023.0074","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/omi.2023.0074","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T23:06:24Z","timestamp":1777676784000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/omi.2023.0074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":66,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1089\/omi.2023.0074"],"URL":"https:\/\/doi.org\/10.1089\/omi.2023.0074","relation":{},"ISSN":["1536-2310","1557-8100"],"issn-type":[{"value":"1536-2310","type":"print"},{"value":"1557-8100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8]]}}}