{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:18:52Z","timestamp":1780582732293,"version":"3.54.1"},"reference-count":38,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Photomedicine and Laser Surgery"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Objectives:<\/jats:italic>\n                    <\/jats:bold>\n                    The aim of this study was to measure the temporal pattern of the expression of osteogenic genes after low-level laser therapy during the process of bone healing. We used quantitative real-time polymerase chain reaction (qPCR) along with histology to assess gene expression following laser irradiation on created bone defects in tibias of rats.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Material and Methods:<\/jats:italic>\n                    <\/jats:bold>\n                    The animals were randomly distributed into two groups: control or laser-irradiated group. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation started 24 h post-surgery and was performed for 3, 6, and 12 sessions, with an interval of 48\u2009h. A 830\u2009nm laser, 50\u2009J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    , 30 mW, was used. On days 7, 13, and 25 post-injury, rats were sacrificed individually by carbon dioxide asphyxia. The tibias were removed for analysis.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    The histological results revealed intense new bone formation surrounded by highly vascularized connective tissue presenting slight osteogenic activity, with primary bone deposition in the group exposed to laser in the intermediary (13 days) and late stages of repair (25 days). The quantitative real-time PCR showed that laser irradiation produced an upregulation of BMP-4 at day 13 post-surgery and an upregulation of BMP4, ALP, and Runx 2 at day 25 after surgery.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic>\n                    <\/jats:bold>\n                    Our results indicate that laser therapy improves bone repair in rats as depicted by differential histopathological and osteogenic genes expression, mainly at the late stages of recovery.\n                  <\/jats:p>","DOI":"10.1089\/pho.2010.2841","type":"journal-article","created":{"date-parts":[[2011,2,9]],"date-time":"2011-02-09T16:18:21Z","timestamp":1297268301000},"page":"311-317","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":67,"title":["Low-Level Laser Therapy Induces Differential Expression of Osteogenic Genes During Bone Repair in Rats"],"prefix":"10.1177","volume":"29","author":[{"given":"Elaine","family":"F\u00e1varo\u2013P\u00edpi","sequence":"first","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Araki","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Uema","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Bossini","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poliani","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivaldo A.","family":"Parizotto","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Tim","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helo\u00edsa Sobreiro Selistre","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Carlos","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Claudia Muniz","family":"Renno","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo","place":["SP\u2013Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,5]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2005.23.161"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2005.01.013"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1002\/lsm.20709","article-title":"In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells, proliferation, growth factors secretion and myogenic differentiation","volume":"40","author":"Hou J.F.","year":"2008","unstructured":"Hou J.F., , Zhang H., , Yuan X., , Li J., , Wei Y.J., , Hu S.S. 2008. 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