{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:18:52Z","timestamp":1777929532403,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Photomedicine and Laser Surgery"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Objective:<\/jats:italic>\n                    <\/jats:bold>\n                    The objective of this study was to determine the effect of light-emitting diode (LED) irradiation on the migration and proliferation of keratinocytes.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Background data:<\/jats:italic>\n                    <\/jats:bold>\n                    Keratinocytes play a key role in re-epithelialization during wound healing; it is speculated that low-level LED therapy might improve keratinocyte migration and proliferation.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Materials and methods:<\/jats:italic>\n                    <\/jats:bold>\n                    Human keratinocyte cells (HKCs) were isolated from child or adult foreskins and irradiated with LED light with a wavelength of 640\u2009nm and a dosage of 12 or 24\u2009J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    . Cell motility, migration, and proliferation were examined using live cell imaging, scratch assay, and a colorimetric cell counting assay, respectively. Hypoxia-inducible factor-1\u03b1 (HIF-1\u03b1) protein levels were analyzed by using Western blotting. Filamentous actin (F-actin) was stained by phalloidin. YC-1 [3-(5-hydroxymethyl-2-furyl)-1-benzylindazole] was used as an HIF-1 inhibitor, and CoCl\n                    <jats:sub>2<\/jats:sub>\n                    (cobalt chloride) and DMOG (dimethyloxaloyl glycine) are HIF-1\u03b1 activators.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    LED irradiation significantly promoted cell motility and migration, but did not significantly influence cell proliferation in HKCs. Furthermore, LED irradiation resulted in a reorganization of cellular F-actin and a dramatic upregulation of HIF-1\u03b1 expression. Suppression of HIF-1\u03b1 using the compound YC-1 prevented reorganization of the actin cytoskeleton following LED irradiation, suggesting that the effect of LED irradiation on the cytoskeleton is mediated through HIF-1\u03b1. Conversely, chemical activation of HIF-1\u03b1 via DMOG or CoCl\n                    <jats:sub>2<\/jats:sub>\n                    resulted in a reorganization of F-actin.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Conclusions:<\/jats:italic>\n                    <\/jats:bold>\n                    LED irradiation may increase keratinocyte migration via HIF-1\u03b1-dependent cytoskeletal reorganization.\n                  <\/jats:p>","DOI":"10.1089\/pho.2015.4077","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T16:02:55Z","timestamp":1464710575000},"page":"313-320","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Light-Emitting Diode Irradiation (640\u2009nm) Regulates Keratinocyte Migration and Cytoskeletal Reorganization Via Hypoxia-Inducible Factor-1\u03b1"],"prefix":"10.1177","volume":"34","author":[{"given":"Chong","family":"Huang","sequence":"first","affiliation":[{"name":"The Second Military Medical University of People's Liberation Army","place":["P.R. 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