{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T07:10:18Z","timestamp":1779433818608,"version":"3.53.1"},"reference-count":49,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Rejuvenation Research"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>\n                    The YiQiFuMai powder injection (YQFM), a traditional Chinese medicine (TCM) prescription re-developed based on the well-known TCM formula Sheng-maisan, showed a wide range of pharmacological activities in cardiovascular diseases in clinics. However, its role in protection against myocardial ischemia\/reperfusion (MI\/R) injury has not been elucidated. The present study not only evaluated the cardioprotective effect of YQFM from MI\/R injury but also investigated the potential molecular mechanisms both\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . The myocardium infarct size, production of lactate dehydrogenase (LDH), creatine kinase (CK), cardiac function, TUNEL staining, and caspase-3 activity were measured. Cell viability was determined, and cell apoptosis was measured by Hoechst 33342 staining and flow cytometry. Mitochondrial membrane potential (\u0394\n                    <jats:italic toggle=\"yes\">\u03a8<\/jats:italic>\n                    m) was measured, and ATP content was quantified by bioluminescent assay. Expression of apoptosis-related proteins, including Caspase-3, Bcl-2, Bax, AMPK\u03b1, and phospho-AMPK\u03b1, was analyzed by western blotting. AMPK\u03b1 siRNA transfection was also applied to the mechanism elucidation. YQFM at a concentration of 1.06\u2009g\/kg significantly reduced myocardium infarct size and the production of LDH, CK in serum, improved the cardiac function, and also produced a significant decrease of apoptotic index. Further, combined treatment with compound C partly attenuated the anti-apoptotic effect of YQFM. In addition, pretreatment with YQFM ranging from 25 to 400\u2009\u03bcg\/mL markedly improved cell viability and decreased LDH release. Moreover, YQFM inhibited H9c2 apoptosis, blocked the expression of caspase-3, and modulated Bcl-2 and Bax proteins, leading to an increased mitochondrial membrane potential and cellular ATP content. Mechanistically, YQFM activated AMP-activated protein kinase (AMPK) signaling pathways whereas pretreatment with AMPK inhibitor Compound C and application of transfection with AMPK\u03b1 siRNA attenuated the anti-apoptotic effect of YQFM. Our results indicated that YQFM could provide significant cardioprotection against MI\/R injury, and potential mechanisms might suppress cardiomyocytes apoptosis, at least in part, through activating the AMPK signaling pathways.\n                  <\/jats:p>","DOI":"10.1089\/rej.2015.1801","type":"journal-article","created":{"date-parts":[[2016,4,12]],"date-time":"2016-04-12T22:03:34Z","timestamp":1460498614000},"page":"495-508","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":20,"title":["YiQiFuMai Powder Injection Attenuates Ischemia\/Reperfusion-Induced Myocardial Apoptosis Through AMPK Activation"],"prefix":"10.1177","volume":"19","author":[{"given":"Fang","family":"Li","sequence":"first","affiliation":[{"name":"China Pharmaceutical University","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianjie","family":"Zheng","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Fan","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kefeng","family":"Zhai","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yisha","family":"Tan","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junping","family":"Kou","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University","place":["P.R. 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