{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:55:08Z","timestamp":1791089708014,"version":"4.1.0"},"reference-count":68,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Rejuvenation Research"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>\n                    Osteoarthritis is a frequent, age-associated disease affecting &gt;10% of world's population over 60 years of age. This study intended to compare intra-articular whole blood clot secretome (autologous conditioned serum [ACS], recently re-named blood clot secretome [BCS]) to platelet-rich plasma (PRP) in knee osteoarthritis (OA). A clinical, nonrandomized open-label comparison of ACS versus PRP in knee OA with subclinical or moderate synovitis symptomology was performed. One hundred and twenty-three patients with knee OA, Kellgren and Lawrence grade II\u2013III, were each treated with six i.a. injections of ACS or PRP. The clinical efficacy was measured by visual analog scale and Western Ontario and McMaster Universities Arthritis Index (WOMAC) score. The biochemical effects measured include synovial fluid (SF) viscosity, cytokines interleukin (IL)-1Ra and IL-1b, radical footprint NO\n                    <jats:sub>3<\/jats:sub>\n                    , and conjugated dienes (CDs). At the 3-month follow-up, clinical efficacy of ACS was significant in all groups, versus PRP. PRP had significant versus baseline efficacy in subclinical, but not in moderate, synovitis cases. ACS was more effective than PRP regarding all analytical parameters. It induced endogenous IL-1Ra expression, downregulated IL-1b, and improved SF viscosity. ACS reduced\u2014significantly stronger than PRP\u2014the concentration of CDs\u2014interpreted as reactive oxygen species footprints\u2014and NO\n                    <jats:sub>3<\/jats:sub>\n                    \u2014interpreted as nitric oxide footprint\u2014in SF. ACS displayed significant efficacy in all groups, which was clinically and biochemically superior to PRP. ACS appears to improve i.a. homeostasis. Strength of this open clinical study is the combination of clinical and biochemical data.\n                  <\/jats:p>","DOI":"10.1089\/rej.2019.2263","type":"journal-article","created":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T02:52:25Z","timestamp":1576637545000},"page":"401-410","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Intra-Articular Injections of a Whole Blood Clot Secretome, Autologous Conditioned Serum, Have Superior Clinical and Biochemical Efficacy Over Platelet-Rich Plasma and Induce Rejuvenation-Associated Changes of Joint Metabolism: A Prospective, Controlled Open-Label Clinical Study in Chronic Knee Osteoarthritis"],"prefix":"10.1177","volume":"23","author":[{"given":"Larisa","family":"Shirokova","sequence":"first","affiliation":[{"name":"Department of Hospital Therapy, Yaroslavl State Medical University of MoH of RF, Yaroslavl, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Noskov","sequence":"additional","affiliation":[{"name":"Department of Hospital Therapy, Yaroslavl State Medical University of MoH of RF, Yaroslavl, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Gorokhova","sequence":"additional","affiliation":[{"name":"Department of Hospital Therapy, Yaroslavl State Medical University of MoH of RF, Yaroslavl, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Reinecke","sequence":"additional","affiliation":[{"name":"ORTHOGEN AG, Dusseldorf, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia","family":"Shirokova","sequence":"additional","affiliation":[{"name":"Department of Hospital Therapy, Yaroslavl State Medical University of MoH of RF, Yaroslavl, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"Osteoarthritis: A serious disease. Osteoarthritis Research Society International 2016. Available from: https:\/\/www.oarsi.org\/sites\/default\/files\/docs\/2016\/oarsi_white_paper_oa_serious_disease_121416_1.pdf. Last accessed August5 2019."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.34453"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-003-1197-1"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmr.2016.06.003"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2165\/00063030-200721050-00004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","unstructured":"Uhlen M Tegel H Sivertsson \u00c5 Kuo C-C Gutierrez JM Lewis NE Forsstr\u00f6m B Dannemeyer M Fagerberg L Malm M Vunk H Edfors F Hober A Sj\u00f6stedt E Kotol D Mulder J Mardinoglu A Schwenk JM Nilsson P Zwahlen M Takanen JO Feilitzen K von Stadler C Lindskog C Pont\u00e9n F Nielsen J Palsson BO Volk A-L Lundqvist M Berling A Svensson A-S Kanje S Enstedt H Afshari D Ekblad S Scheffel J Katona B Vuu J Lindstr\u00f6m E Xu L Mihai R Bremer L Westin M Muse M Mayr LM Knight S G\u00f6pel S Davies R Varley P Hatton D Fields R Voldborg BG Rockberg J Schiavone LH Hober S. The human secretome\u2014The proteins secreted from human cells. bioRxiv 2018:465815; DOI: 10.1101\/465815. Available from: https:\/\/www.biorxiv.org\/content\/early\/2018\/11\/26\/465815#disqus_thread. Last accessed September5 2019.","DOI":"10.1101\/465815"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7309031"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2008.06.014"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145551"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e3181076514"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.5604\/15093492.1198829"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-821303"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-821304"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/0363546509348047"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-019-05465-8"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.11152\/mu-1495"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-011-1458-4"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00699"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/2040622319825567"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1051\/sicotj\/2017036"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1177\/1947603516670709"},{"key":"e_1_3_3_23_2","first-page":"502","article-title":"Efficacy of platelet-rich plasma as conservative treatment in orthopaedics: A systematic review and meta-analysis","volume":"16","author":"Franchini M","year":"2018","unstructured":"Franchini M, , Cruciani M, , Mengoli C, , Marano G, , Pupella S, , Veropalumbo E, , Masiello F, , Pati I, , Vaglio S, , Liumbruno GM. Efficacy of platelet-rich plasma as conservative treatment in orthopaedics: A systematic review and meta-analysis. Blood Transfus, 2018; 16:502\u2013513.","journal-title":"Blood Transfus"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.17.2.229"},{"key":"e_1_3_3_25_2","first-page":"68","article-title":"Structure application in the complex treatment of the pain syndrome in the lower back (in Russian)","volume":"8","author":"Mazurov VI","year":"2004","unstructured":"Mazurov VI, , Belyaeva IB. Structure application in the complex treatment of the pain syndrome in the lower back (in Russian). Ther Arch, 2004; 8:68\u201371.","journal-title":"Ther Arch"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","unstructured":"Repetto M Semprine J Boveris A. Lipid peroxidation: Chemical mechanism biological implications and analytical determination. Lipid Peroxidation Angel Catala IntechOpen. 2012. Available from: https:\/\/www.intechopen.com\/books\/lipid-peroxidation\/lipid-peroxidation-chemical-mechanism-biological-implications-and-analytical-determination Last accessed January20 2020.","DOI":"10.5772\/45943"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.22237\/jmasm\/1257035100"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2004.022905"},{"issue":"2","key":"e_1_3_3_29_2","first-page":"108","article-title":"Impiego Di Siero Autologo Condizionato (ACS\u2014Orthokine) In Pazienti Affetti Da Gonartrosi Resistenti Al Trattamento Con Plasma Ricco Di Piastrine","volume":"17","author":"Leone R","year":"2019","unstructured":"Leone R, , DeRosa A, , Iudicone P, , Fioravanti D, , Capua G, , Rossetti F, , Lavorino C, , Costantino S, , Pierelli L. Impiego Di Siero Autologo Condizionato (ACS\u2014Orthokine) In Pazienti Affetti Da Gonartrosi Resistenti Al Trattamento Con Plasma Ricco Di Piastrine. [Use of conditioned autologous serum (ACS\u2014Orthokine) in patients with gonarthrosis resistant to treatment with plasma rich in platelets.] Blood Transfus, 2019; 17 Suppl 2:108.","journal-title":"[Use of conditioned autologous serum (ACS\u2014Orthokine) in patients with gonarthrosis resistant to treatment with plasma rich in platelets.] Blood Transfus"},{"key":"e_1_3_3_30_2","first-page":"30","article-title":"Direct antioxidant properties of bilirubin and biliverdin","volume":"3","author":"Jansen T","year":"2012","unstructured":"Jansen T, , Daiber A. Direct antioxidant properties of bilirubin and biliverdin. is there a role for biliverdin reductase? Front Pharmacol, 2012; 3:30.","journal-title":"is there a role for biliverdin reductase? Front Pharmacol"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.07.004"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2018.11.006"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/573528"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aan1471"},{"key":"e_1_3_3_35_2","unstructured":"Platelet Rich Plasma (PRP) Injection. Information and instructions for patients. Standord School of Medicine. Website of Stanford University Medical Center Department of Radiology. p. 3. Available from: https:\/\/stanfordhealthcare.org\/content\/dam\/SHC\/diagnosis\/p\/docs\/petctscan-pdf-prpbeaulieuletter.pdf. Last accessed January20 2020."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2010.01.020"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-11-220"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02345083"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2005.01203.x"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2003.12.010"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24322"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.07.020"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.14.3.305"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.32.1.29"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1177\/0363546515582027"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17071064"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.16.01374"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.asmart.2016.11.003"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1177\/0363546517746112"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.3.290"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380515"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2018.19.6.735"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm050867v"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.08.011"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keu016"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2016.11.005"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.213"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.09.008"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1993.tb15137.x"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401915"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(96)80009-4"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10056"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.39006"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-019-1923-x"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.04.010"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12562"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0092-9"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.04.007"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2017.02.756"}],"container-title":["Rejuvenation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/rej.2019.2263","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/rej.2019.2263","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/rej.2019.2263","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T01:35:04Z","timestamp":1777685704000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/rej.2019.2263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":68,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1089\/rej.2019.2263"],"URL":"https:\/\/doi.org\/10.1089\/rej.2019.2263","relation":{},"ISSN":["1549-1684","1557-8577"],"issn-type":[{"value":"1549-1684","type":"print"},{"value":"1557-8577","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10]]}}}