{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:16:41Z","timestamp":1777933001928,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Rejuvenation Research"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:p>NQO1 disruption enhances susceptibility to oxidative stress during hyperglycemia and is a significant contributor to the development and progression of diabetes. Oxidative stress has been linked to several symptoms, including hyperglycemia, reactive oxygen species buildup, high blood pressure, and the expression of inflammatory markers. Therefore, the present research aimed to evaluate the genetic abnormality of NQO1 (rs1800566, C609T) gene polymorphism, expression, and vitamin-D level assessment among Type 2 diabetes mellitus (T2DM) patients. The study included 100 newly diagnosed T2DM cases and 100 healthy individuals as healthy controls. Total RNA was extracted from the whole blood using the TRIzol method, and further cDNA was synthesized, and expression was evaluated.<\/jats:p>\n                  <jats:p>\n                    There is a significant difference in NQO1 (rs1800566, C609T) genotype distribution among the T2DM patients and healthy controls (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    = 0.04). Compared with the NQO1 CC wild-type genotype, the NQO1 CT heterozygous genotype had an odds ratio of 1.96 (1.08\u20133.55), and the NQO1 TT mutant type genotype had an odds ratio of 3.31 (0.61\u201317.77). Significantly decreased expression of NQO1 mRNA was observed with heterozygous CT (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.0001) and homozygous mutant TT genotype (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    = 0.0004), compared with homozygous wild-type CC genotype. NQO1 mRNA expression level was also compared with vitamin D levels among the T2DM patients. T2DM patients with vitamin D deficiency had 1.83-fold NQO1 mRNA expression, while vitamin D insufficient and sufficient T2DM cases had 3.31-fold (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.0001) and 3.70-fold (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.0001) NQO1 mRNA expression.\n                  <\/jats:p>\n                  <jats:p>It was concluded that NQO1 (rs1800566, C609T) CT and TT genotypes played a significant role in the worseness of type II diabetes mellitus, and decreased expression of NQO1 mRNA expression could be an essential factor for disease worseness as well as hypermethylation could be a factor for reduced expression leading to disease severity. The decreased NQO1 mRNA expression with heterozygous CT and mutant TT genotype associated with vitamin D deficiency may contribute to disease progression.<\/jats:p>","DOI":"10.1089\/rej.2024.0032","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T12:40:02Z","timestamp":1720010402000},"page":"145-153","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Role of NQO1 Gene Involvement and Susceptibility of T2DM Among Saudi Arabia Population"],"prefix":"10.1177","volume":"27","author":[{"given":"Jwaher Haji","family":"Alhaji","sequence":"first","affiliation":[{"name":"Department of Health Sciences, College of Applied Studies and Community Service, King Saud University, Riyadh, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Divya","family":"Pathak","sequence":"additional","affiliation":[{"name":"Central Drugs Standard Control Organisation, New Delhi, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fauzia","family":"Ashfaq","sequence":"additional","affiliation":[{"name":"Clinical Nutrition Department, Applied Medical Sciences College, Jazan University, Jazan, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulrahman A.","family":"Alsayegh","sequence":"additional","affiliation":[{"name":"Clinical Nutrition Department, Applied Medical Sciences College, Jazan University, Jazan, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahmida","family":"Khatoon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, University of Ha\u2019il, Ha\u2019il, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bader Judaya","family":"Almutairi","sequence":"additional","affiliation":[{"name":"Clinical Nutrition, Al-Rass General Hospital, Ministry of Health, Qassim, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Idreesh","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirza Masroor Ali","family":"Beg","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Alatoo International University, Bishkek, Kyrgyzstan."},{"name":"Center for Promotion of Medical Research, Alatoo International University, Bishkek, 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