{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T18:10:11Z","timestamp":1783793411300,"version":"3.55.0"},"reference-count":57,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,5,25]],"date-time":"2011-05-25T00:00:00Z","timestamp":1306281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2011,5,25]],"date-time":"2011-05-25T00:00:00Z","timestamp":1306281600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n                    Dupuytren's disease (DD) is a fibroproliferative disorder characterized by aberrant proliferation of myofibroblasts, the source of which remains unknown. Recent studies indicate that circulating and tissue-resident mesenchymal stem cells (MSCs) can differentiate into myofibroblasts. Therefore, the aim of this study was to profile MSCs from phenotypically distinct DD sites including cord, nodule, skin overlying nodule (SON), and perinodular fat (PNF) compared with unaffected internal controls, that is, distant palmar fat (DPF) and transverse palmar fascia (Skoog's fibers) as well as external control carpal tunnel (CT) tissue including skin, fat, and fascia. Freshly isolated primary fibroblasts as well as cells grown up to passage 5 (P5) from DD (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =27) and CT (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =14) samples were analyzed for the presence of established MSC markers CD73, CD90, and CD105 and absence of hematopoietic marker CD34 using fluorescence-activated cell sorting, in-cell quantitative western blotting, immunohistochemistry, and immunocytochemistry. Freshly isolated cells from SON, PNF, and cord biopsies had a higher number of CD34\n                    <jats:sup>\u2212<\/jats:sup>\n                    73\n                    <jats:sup>+<\/jats:sup>\n                    90\n                    <jats:sup>+<\/jats:sup>\n                    105\n                    <jats:sup>+<\/jats:sup>\n                    cells compared with Skoog's fibers and CT controls. P3 cells obtained from all DD biopsies compared with CT samples differentiated into osteocytes, adipocytes, and chondrocytes. P3 cord and nodule cells expressed intense \u03b1-smooth muscle actin staining compared with skin and fat cells. Stem cell markers including stem cell factor, MSC-homing marker\n                    <jats:italic toggle=\"yes\">CXCR4<\/jats:italic>\n                    , and Wnt\/\u03b2-catenin downregulator Dkk-1 were all upregulated in SON and PNF compared with CT skin and CT fat, respectively, as shown by real-time quantitative polymerase chain reaction. However, osteogenic marker\n                    <jats:italic toggle=\"yes\">OSF-1<\/jats:italic>\n                    had a significantly higher expression in the PNF (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.002) and cord (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.01) compared with the nodule. In conclusion, we have shown the presence of MSCs in specific DD tissue phenotypes compared with internal and external control tissue. These findings provide preliminary support for a potential alternative source of disease myofibroblasts originating from sites such as SON and PNF as opposed to palmar fascia alone.\n                  <\/jats:p>","DOI":"10.1089\/scd.2011.0140","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T02:16:31Z","timestamp":1306376191000},"page":"609-622","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":29,"title":["Identification of Mesenchymal Stem Cells in Perinodular Fat and Skin in Dupuytren's Disease: A Potential Source of Myofibroblasts with Implications for Pathogenesis and Therapy"],"prefix":"10.1177","volume":"21","author":[{"given":"Syed Amir","family":"Iqbal","sequence":"first","affiliation":[{"name":"University of Manchester","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Manning","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhatullah","family":"Syed","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatesh","family":"Kolluru","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Hayton","sequence":"additional","affiliation":[{"name":"Wrightington Hospital","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart","family":"Watson","sequence":"additional","affiliation":[{"name":"South Manchester University Hospital Foundation Trust","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ardeshir","family":"Bayat","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["United Kingdom"]},{"name":"South Manchester University Hospital Foundation Trust","place":["United Kingdom"]},{"name":"University of Manchester","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,5,25]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.180"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11552-008-9160-9"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.12968\/hmed.2007.68.11.27683"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1308\/003588406X83104"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/J.JHSB.2006.03.174"},{"key":"e_1_3_3_7_2","first-page":"106","article-title":"Partial fasciectomy for Dupuytren's contractures","volume":"18","author":"Mavrogenis AF","year":"2009","unstructured":"Mavrogenis AF, , Spyridonos SG, , Ignatiadis IA, , Antonopoulos D, , Papagelopoulos PJ. 2009. 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