{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:24:28Z","timestamp":1777933468253,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2011,9,26]],"date-time":"2011-09-26T00:00:00Z","timestamp":1316995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>\n                    DEK is a biochemically distinct protein that is generally found in the nucleus, where it is vital to global heterochromatin integrity. However, DEK is also secreted by cells (eg, macrophages) and influences other adjacent cells (eg, acts as a chemoattractant for certain mature blood cells). We hypothesized that DEK may modulate functions of hematopoietic stem (HSCs) and progenitor (HPCs) cells. C57Bl\/6 mice were used to demonstrate that absolute numbers and cycling status of HPCs (colony forming unit-granulocyte macrophage [CFU-GM], burst forming unit-erythroid [BFU-E], and colony forming unit-granulocyte erythroid macrophage megakaryocyte [CFU-GEMM]) in bone marrow (BM) and spleen were significantly enhanced in DEK \u2212\/\u2212 as compared with wild-type (WT) control mice. Moreover, purified recombinant DEK protein inhibited colony formation in vitro by CFU-GM, BFU-E, and CFU-GEMM from WT BM cells and human cord blood (CB) cells in a dose-dependent fashion, demonstrating that DEK plays a negative role in HPC proliferation in vitro and in vivo. Suppression was direct acting as determined by inhibition of proliferation of single isolated CD34\n                    <jats:sup>+<\/jats:sup>\n                    CB cells in vitro. In contrast, DEK \u2212\/\u2212 BM cells significantly demonstrated reduced long term competitive and secondary mouse repopulating HSC capacity compared with WT BM cells, demonstrating that DEK positively regulates engrafting capability of self-renewing HSCs. This demonstrates that DEK has potent effects on HSCs, HPCs, and hematopoiesis, information of biological and potential clinical interest.\n                  <\/jats:p>","DOI":"10.1089\/scd.2011.0451","type":"journal-article","created":{"date-parts":[[2011,9,27]],"date-time":"2011-09-27T01:44:01Z","timestamp":1317087841000},"page":"1449-1454","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":31,"title":["DEK Regulates Hematopoietic Stem Engraftment and Progenitor Cell Proliferation"],"prefix":"10.1177","volume":"21","author":[{"given":"Hal E.","family":"Broxmeyer","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferdinand","family":"Kappes","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center"},{"name":"RWTH Aachen University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirit","family":"Mor-Vaknin","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Legendre","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Kinzfogl","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Cooper","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giao","family":"Hangoc","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Markovitz","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,9,26]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"253","volume-title":"Hematology: Basic Principles and Practice","author":"Shaheen M","year":"2009","unstructured":"Shaheen M, , Broxmeyer HE. 2009. The humoral regulation of hematopoiesis. Hematology: Basic Principles and Practice, 5th Hoffman R, , Benz EJJr. Shattil SJ, , Furie B, , Silberstein LE, , McGlave P, , Heslop H, , Anastasi J. Elsevier Churchill Livingston: Philadelphia, PAPart III, Chapter 24253\u2013275.","edition":"5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.08.029"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2036411"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01030-06"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.30138"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-1063"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-2330"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2304"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.2"},{"key":"e_1_3_3_11_2","first-page":"6011","article-title":"Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK Mol","volume":"24","author":"Kappes F","year":"2004","unstructured":"Kappes F, , Damoc C, , Knippers R, , Przybylski M, , Pinna LA, , Gruss C. 2004. Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK Mol. Cell Biol, 24:6011\u20136020.","journal-title":"Cell Biol"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041385"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-325381"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-195214"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1097071"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500884200"}],"container-title":["Stem Cells and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/scd.2011.0451","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/scd.2011.0451","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/scd.2011.0451","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T01:46:44Z","timestamp":1777686404000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/scd.2011.0451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,26]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1089\/scd.2011.0451"],"URL":"https:\/\/doi.org\/10.1089\/scd.2011.0451","relation":{},"ISSN":["1547-3287","1557-8534"],"issn-type":[{"value":"1547-3287","type":"print"},{"value":"1557-8534","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,26]]}}}