{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:37:11Z","timestamp":1791221831580,"version":"4.1.0"},"reference-count":23,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2012,1,5]],"date-time":"2012-01-05T00:00:00Z","timestamp":1325721600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>\n                    Dogs provide a more clinically relevant model of human disease than rodents, particularly with respect to hereditary diseases. Thus, the availability of canine stem cells will greatly facilitate the use of the dog in the development of stem cell-based gene therapies and regenerative medicine. In this study we describe the production of canine induced pluripotent stem cells (ciPSCs) from adult dermal fibroblasts. These cells have a morphology resembling previously described canine embryonic stem cells, a normal karyotype, and express pluripotency markers including alkaline phosphatase,\n                    <jats:italic toggle=\"yes\">Nanog<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Oct4<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Telomerase<\/jats:italic>\n                    , SSEA1, SSEA4, TRA1-60, TRA1-81, and Rex1. Furthermore, the inactive X chromosome is reactivated indicating a ground-state pluripotency. In culture they readily form embryoid bodies, which in turn give rise to cell types from all 3 embryonic germ layers, as indicated by expression of the definitive endoderm markers\n                    <jats:italic toggle=\"yes\">Cxcr4<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">\u03b1-fetoprotein<\/jats:italic>\n                    , mesoderm markers\n                    <jats:italic toggle=\"yes\">Collagen IIA<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Gata2<\/jats:italic>\n                    , and ectoderm markers\n                    <jats:italic toggle=\"yes\">\u03b2III-tubulin<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Enolase<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Nestin<\/jats:italic>\n                    . Of particular significance is the observation that these ciPSCs are dependent only on leukemia inhibitory factor (LIF), making them similar to mouse and canine embryonic stem cells, but strikingly unlike the ciPSCs recently described in two other studies, which were dependent on both basic fibroblast growth factor and LIF in order to maintain their pluripotency. Thus, our ciPSCs closely resemble mouse ESCs derived from the inner cell mass of preimplantation embryos, while the previously described ciPSCs appear to be more representative of cells from the epiblast of mouse postimplantation embryos.\n                  <\/jats:p>","DOI":"10.1089\/scd.2011.0608","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T03:00:29Z","timestamp":1325818829000},"page":"2288-2297","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":70,"title":["Generation and Characterization of LIF-dependent Canine Induced Pluripotent Stem Cells from Adult Dermal Fibroblasts"],"prefix":"10.1177","volume":"21","author":[{"given":"Deanne J.","family":"Whitworth","sequence":"first","affiliation":[{"name":"University of Queensland","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A.","family":"Ovchinnikov","sequence":"additional","affiliation":[{"name":"University of Queensland","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst J.","family":"Wolvetang","sequence":"additional","affiliation":[{"name":"University of Queensland","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,1,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.024"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1071\/RD07039"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1071\/RD09270"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810387939"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04338"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.235739"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2011.0127"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21117"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20392"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0357"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0640"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0433"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2008.0336"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05950"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05972"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.01.003"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09228"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.08.035"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2011.01148.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022413"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.7.4803"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-092910-154020"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-011-1024-7"}],"container-title":["Stem Cells and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/scd.2011.0608","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/scd.2011.0608","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/scd.2011.0608","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T01:47:04Z","timestamp":1777686424000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/scd.2011.0608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,5]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1089\/scd.2011.0608"],"URL":"https:\/\/doi.org\/10.1089\/scd.2011.0608","relation":{},"ISSN":["1547-3287","1557-8534"],"issn-type":[{"value":"1547-3287","type":"print"},{"value":"1557-8534","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,5]]}}}