{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:13:30Z","timestamp":1791108810488,"version":"4.1.0"},"reference-count":52,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2012,12,18]],"date-time":"2012-12-18T00:00:00Z","timestamp":1355788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>\n                    The bone marrow (BM) microenvironment has clearly been implicated in the pathogenesis of B-cell chronic lymphocytic leukemia (B-CLL). However, the potential involvement of BM stromal progenitors, the mesenchymal stem cells (MSCs), in the pathophysiology of the disease has not been extensively investigated. We expanded in vitro BM-MSCs from B-CLL patients (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =11) and healthy individuals (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =16) and comparatively assessed their reserves, proliferative potential, differentiation capacity, and immunoregulatory effects on T- and B-cells. We also evaluated the anti-apoptotic effect of patient-derived MSCs on leukemic cells and studied their cytogenetic characteristics in comparison to BM hematopoietic cells. B-CLL-derived BM MSCs exhibit a similar phenotype, differentiation potential, and ability to suppress T-cell proliferative responses as compared with MSCs from normal controls. Furthermore, they do not carry the cytogenetic abnormalities of the leukemic clone, and they exert a similar anti-apoptotic effect on leukemic cells and healthy donor-derived B-cells, as their normal counterparts. On the other hand, MSCs from B-CLL patients significantly promote normal B-cell proliferation and IgG production, in contrast to healthy-donor-derived MSCs. Furthermore, they have impaired reserves, defective cellular growth due to increased apoptotic cell death and exhibit aberrant production of stromal cell-derived factor 1, B-cell activating factor, a proliferation inducing ligand, and transforming growth factor \u03b21, cytokines that are crucial for the survival\/nourishing of the leukemic cells. We conclude that ex vivo expanded B-CLL-derived MSCs harbor intrinsic qualitative and quantitative abnormalities that may be implicated in disease development and\/or progression.\n                  <\/jats:p>","DOI":"10.1089\/scd.2012.0255","type":"journal-article","created":{"date-parts":[[2012,12,19]],"date-time":"2012-12-19T00:30:28Z","timestamp":1355877028000},"page":"1329-1341","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Study of the Quantitative, Functional, Cytogenetic, and Immunoregulatory Properties of Bone Marrow Mesenchymal Stem Cells in Patients with B-Cell Chronic Lymphocytic Leukemia"],"prefix":"10.1177","volume":"22","author":[{"given":"Charalampos","family":"Pontikoglou","sequence":"first","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Christina","family":"Kastrinaki","sequence":"additional","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Klaus","sequence":"additional","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Kalpadakis","sequence":"additional","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavlos","family":"Katonis","sequence":"additional","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalliopi","family":"Alpantaki","sequence":"additional","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerassimos A.","family":"Pangalis","sequence":"additional","affiliation":[{"name":"Athens Medical Center-Psychikon Branch","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen A.","family":"Papadaki","sequence":"additional","affiliation":[{"name":"University of Crete School of Medicine","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,12,18]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041720"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04679.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.00305.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.7.2387"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.8.2655"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.5.1795.h81702001795_1795_1801"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403544"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07868.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-07-233718"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.031377"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2006.11.024"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-10-246199"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2011.00164"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-05-352492"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-011-9228-8"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.136"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0345"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23119"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.559"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/1042819021000029993"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2010.09.006"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.029736"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.052779"},{"key":"e_1_3_3_26_2","first-page":"67","volume-title":"Methods in Molecular Medicine","author":"Delorme B","year":"2007","unstructured":"Delorme B, , Charbord P. 2007. 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