{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:40:59Z","timestamp":1791862859977,"version":"4.3.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T00:00:00Z","timestamp":1409097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>Tissue resident stem cells are believed to exist in every organ, and their identification is commonly done using a combination of immunostaining for putative stem cell markers and label-retaining cell (LRC) strategy. In this study, we employed these approaches to identify potential stem cells in the penis. Newborn rats were intraperitoneally injected with thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), and their penis was harvested at 7\u2009h, 3 days, 1 week, and 4 weeks. It was processed for EdU stains and immunofluorescence staining for stem cell markers A2B5, PCNA, and c-kit. EdU-positive cells were counted for each time point and co-localized with each stem cell marker, then isolated and cultured in vitro followed by their characterization using flowcytometry and immunofluorescence. At 7\u2009h post-EdU injection, 410\u00b1105.3 penile corporal cells were labeled in each cross-section (\u223c28%). The number of EdU-positive cells at 3 days increased to 536\u00b1115.6, while their percentage dropped to 25%. Progressively fewer EdU-positive cells were present in the sacrificed rat penis at longer time points (1 and 4 weeks). They were mainly distributed in the subtunic and perisinusoidal spaces, and defined as subtunic penile progenitor cells (STPCs) and perisinusoidal penile progenitor cells (PPCs). These cells expressed c-kit, A2B5, and PCNA. After culturing in vitro, only \u223c0.324% corporal cells were EdU-labeled LRCs and expressed A2B5\/PCNA. Therefore, labeling of penis cells by EdU occurred randomly, and label retaining was not associated with expression of c-kit, A2B5, or PCNA. The penile LRCs are mainly distributed within the subtunic and perisinusoidal space.<\/jats:p>","DOI":"10.1089\/scd.2014.0360","type":"journal-article","created":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T18:35:54Z","timestamp":1409164554000},"page":"264-270","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":25,"title":["Presence of Stem\/Progenitor Cells in the Rat Penis"],"prefix":"10.1177","volume":"24","author":[{"given":"Guiting","family":"Lin","sequence":"first","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amjad","family":"Alwaal","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of California"},{"name":"Peking University First Hospital","place":["People's Republic of China"]},{"name":"Peking University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwen","family":"Wang","sequence":"additional","affiliation":[{"name":"University of California"},{"name":"Capital Medical University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"University of California"},{"name":"Peking University First Hospital","place":["People's Republic of China"]},{"name":"Peking University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixi","family":"Li","sequence":"additional","affiliation":[{"name":"University of California"},{"name":"Peking University First Hospital","place":["People's Republic of China"]},{"name":"Peking University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifang","family":"Wang","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxiu","family":"Ning","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Shwun","family":"Lin","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongcheng","family":"Xin","sequence":"additional","affiliation":[{"name":"University of California"},{"name":"Peking University First Hospital","place":["People's Republic of China"]},{"name":"Peking University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom F.","family":"Lue","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,8,27]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0901-778"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0134"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2005.14.337"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.041038"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2008.07507.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3507"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00533.2007"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4163"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.8.2035"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/14653240903180084"},{"key":"e_1_3_3_12_2","first-page":"746e1","article-title":"Label retaining and stem cell marker expression in the developing rat urinary bladder","volume":"3","author":"Zhang H","year":"2011","unstructured":"Zhang H, , Lin G, , Qiu X, , Ning H, , Banie L, , Lue TF, and Lin CS. 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