{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:30:59Z","timestamp":1777933859142,"version":"3.51.4"},"reference-count":69,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>\n                    The spin-trap free radical scavenger\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    -tert-butyl-\u03b1-phenylnitron (PBN) ameliorated effects of several teratogens involving reactive oxygen species (ROS). We investigated for the first time whether PBN could ameliorate teratogenesis induced by a DNA hypomethylating hematological therapeutic 5-azacytidine (5azaC). At days 12 and 13 of gestation, Fisher rat dams were pretreated by an i.v. injection of PBN (40\u2009mg\/kg) and 1\u2009h later by an i.p. injection of 5azaC (5mg\/kg). Development was analyzed at gestation day 15 in embryos and day 20 in fetuses. PBN alone did not significantly affect development. PBN pretreatment restored survival of 5azaC-treated dams' embryos to the control level, restored weight of embryos and partially of fetuses, and partially restored crown-rump lengths. PBN pretreatment converted limb adactyly to less severe oligodactyly. PBN pretreatment restored global DNA methylation level in the limb buds to the control level. Cell proliferation in limb buds of all 5azaC-treated dams remained significantly lower than in controls. In the embryonic liver, PBN pretreatment normalized proliferation diminished significantly by 5azaC; whereas in embryonic vertebral cartilage, proliferation of all 5azaC-treated dams was significantly higher than in PBN-treated dams or controls. Apoptotic indices significantly enhanced by 5azaC in liver and cartilage were not influenced by PBN pretreatment. However, PBN significantly diminished ROS or reactive nitrogen species markers nitrotyrosine and 8-hydroxy-2\u2032deoxyguanosine elevated by 5azaC in embryonic tissues, and, therefore, activity of this DNA hypomethylating agent was associated to the activation of free radicals. That pretreatment with PBN enhanced proliferation in the liver and not in immature tissue is interesting for the treatment of 5azaC-induced hepatotoxicity and liver regeneration.\n                  <\/jats:p>","DOI":"10.1089\/scd.2018.0194","type":"journal-article","created":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T06:29:03Z","timestamp":1548224943000},"page":"717-733","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["A Free Radical Scavenger Ameliorates Teratogenic Activity of a DNA Hypomethylating Hematological Therapeutic"],"prefix":"10.1177","volume":"28","author":[{"given":"Nikola","family":"Sobo\u010dan","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, School of Medicine, University Hospital Merkur, University of Zagreb, Zagreb, Croatia."},{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Katu\u0161i\u0107 Bojanac","sequence":"additional","affiliation":[{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."},{"name":"Department of Medical Biology, School of Medicine, University of Zagreb, Zagreb, Croatia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nino","family":"Sin\u010di\u0107","sequence":"additional","affiliation":[{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."},{"name":"Department of Medical Biology, School of Medicine, University of Zagreb, Zagreb, Croatia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Himelreich-Peri\u0107","sequence":"additional","affiliation":[{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."},{"name":"Department of 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Zagreb, Zagreb, Croatia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ljiljana","family":"\u0160erman","sequence":"additional","affiliation":[{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."},{"name":"Department of Medical Biology, School of Medicine, University of Zagreb, Zagreb, Croatia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Vlahovi\u0107","sequence":"additional","affiliation":[{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."},{"name":"Department of Medical Biology, School of Medicine, University of Zagreb, Zagreb, Croatia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davor","family":"Je\u017eek","sequence":"additional","affiliation":[{"name":"Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia."},{"name":"Department of Histology and 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