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However, it is unclear whether the standard chondrogenic medium using transforming growth factor beta (TGF\u03b2) isoforms is optimal to differentiate these cells. We therefore used pellet culture to screen progenitors from immature bovine articular cartilage with a number of chondrogenic factors and discovered that bone morphogenetic protein-9 (BMP9) precociously induces their differentiation. This difference was apparent with toluidine blue staining and confirmed by biochemical and transcriptional analyses with BMP9-treated progenitors exhibiting 11-fold and 5-fold greater aggrecan and collagen type II (COL2A1) gene expression than TGF\u03b21-treated progenitors. Quantitative gene expression analysis over 14 days highlighted the rapid and phased nature of BMP9-induced chondrogenesis with sequential activation of aggrecan then collagen type II, and negligible collagen type X gene expression. The extracellular matrix of TGF\u03b21-treated progenitors analyzed using atomic force microscopy was fibrillar and stiff whist BMP9-induced matrix of cells more compliant and correspondingly less fibrillar. Polarized light microscopy revealed an annular pattern of collagen fibril deposition typified by TGF\u03b21-treated pellets, whereas BMP9-treated pellets displayed a birefringence pattern that was more anisotropic. Remarkably, differentiated immature chondrocytes incubated as high-density cultures in vitro with BMP9 generated a pronounced anisotropic organization of collagen fibrils indistinguishable from mature adult articular cartilage, with cells in deeper zones arranged in columnar manner. This contrasted with cells grown with TGF\u03b21, where a concentric pattern of collagen fibrils was visualized within tissue pellets. In summary, BMP9 is a potent chondrogenic factor for articular cartilage progenitors and is also capable of inducing morphogenesis of adult-like cartilage, a highly desirable attribute for in vitro tissue-engineered cartilage.<\/jats:p>","DOI":"10.1089\/scd.2019.0209","type":"journal-article","created":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T05:21:45Z","timestamp":1588396905000},"page":"882-894","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":33,"title":["Bone Morphogenetic Protein-9 Is a Potent Chondrogenic and Morphogenic Factor for Articular Cartilage Chondroprogenitors"],"prefix":"10.1177","volume":"29","author":[{"given":"Ben J.","family":"Morgan","sequence":"first","affiliation":[{"name":"Centre of Nanohealth, Swansea University Medical School, Swansea, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Bauza-Mayol","sequence":"additional","affiliation":[{"name":"Centre of Nanohealth, Swansea University Medical School, Swansea, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver F. 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