{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:29:10Z","timestamp":1777933750113,"version":"3.51.4"},"reference-count":52,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    Recently, cell therapy has been developed as a novel treatment for perinatal hypoxic-ischemic encephalopathy (HIE), which is an important cause of neurological disorder and death, and stem cells from human exfoliated deciduous teeth (SHED) express early markers for mesenchymal and neuroectodermal stem cells. We investigated the treatment effect of SHED for HIE in neonatal rats. Seven-day-old rats underwent ligation of the left carotid artery and were exposed to 8% hypoxic treatment. SHED (1\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    cells) were injected via the right external jugular vein 24\u2009h after the insult. The effect of intravenous administration of SHED cells was evaluated neurologically and pathophysiologically. In the evaluation of engraftment using quantum dots 655, only a few SHED were detected in the injured cortex. In the immunohistological evaluation 24\u2009h after injection, the numbers of positive cells of active caspase-3 and anti-4 hydroxynonenal antiserum were lower in the SHED group than in the vehicle group. The number of Iba-1\n                    <jats:sup>+<\/jats:sup>\n                    cells in the cortex was higher in the SHED group. However, the proportion of M1 microglia (Iba-1\n                    <jats:sup>+<\/jats:sup>\n                    \/ED-1\n                    <jats:sup>+<\/jats:sup>\n                    ) was significantly decreased, whereas M2 microglia (Iba-1\n                    <jats:sup>+<\/jats:sup>\n                    \/CD206\n                    <jats:sup>+<\/jats:sup>\n                    ) tended to increase in the SHED group. In the behavioral tests performed 5 months after hypoxic treatment, compared to the vehicle group, the SHED group showed significant elongation of the endurance time in the rotarod treadmill test, significantly ameliorated proportion of using the impaired hand in the cylinder test, significantly lower ratio of right\/left front paw area in gait analysis, and significantly higher avoidance rate in the active avoidance test. In the in vitro experiment with cultured neurons exposed to oxygen-glucose deprivation, we confirmed the neuroprotective effect of the condition medium of SHED. These results suggested that intravenous administration of SHED exerted a treatment effect both histologically and functionally, possibly via a paracrine effect.\n                  <\/jats:p>","DOI":"10.1089\/scd.2019.0221","type":"journal-article","created":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:46:17Z","timestamp":1575506777000},"page":"63-74","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["A Novel Treatment with Stem Cells from Human Exfoliated Deciduous Teeth for Hypoxic-Ischemic Encephalopathy in Neonatal Rats"],"prefix":"10.1177","volume":"29","author":[{"given":"Yuma","family":"Kitase","sequence":"first","affiliation":[{"name":"Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital, Nagoya, Japan."},{"name":"Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuto","family":"Ueda","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alkisti","family":"Mikrogeorgiou","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichiro","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohki","family":"Matsubara","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Tsukagoshi Okabe","sequence":"additional","affiliation":[{"name":"Department of Advanced Medicine, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinobu","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Advanced Medicine, Nagoya University Hospital, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Hand Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Yukawa","sequence":"additional","affiliation":[{"name":"Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Nagoya, Japan."},{"name":"Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan."},{"name":"Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinobu","family":"Baba","sequence":"additional","affiliation":[{"name":"Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Nagoya, 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