{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T04:48:49Z","timestamp":1793249329239,"version":"4.5.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>Pressure ulcers (PUs) result in part due to ischemia\u2013reperfusion injury to the skin and present frequently in elderly or quadriplegic patients with reduced mobility. Despite the high economic and societal cost of this condition, PU therapy relies primarily on preventive strategies and invasive surgical intervention. A growing body of clinical literature suggests that localized injection of adipose-derived cells can accelerate and enhance the closure of PUs. The current study systematically evaluated the safety of human adipose stromal vascular fraction (SVF) cells isolated using a closed system device when injected into a murine PU injury model. The human SVF cells were characterized by colony-forming unit\u2013fibroblast and differentiation assays before use. Young (2 months) immunocompetent C57BL\/6 mice subjected to a magnet-induced ischemia\u2013reperfusion injury were injected subcutaneously with human SVF cells at increasing doses (0.25\u20132 million cells). The size of the PU was monitored over a 20-day period. Both female and male mice tolerated the concentration-dependent injection of the SVF cells without complications. While male mice trended toward more rapid wound closure rates in response to lower SVF cell concentrations (0.25\u20130.5 million cells), female mice responded favorably to higher SVF cell concentrations (1\u20132 million cells); however, outcomes did not reach statistical significance in either sex. Overall, the study demonstrates that human SVF cells prepared with a closed system device designed for use at point of care can be safely administered for PU therapy in an immunocompetent host animal model.<\/jats:p>","DOI":"10.1089\/scd.2019.0245","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T01:26:47Z","timestamp":1580261207000},"page":"452-461","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Safety of Human Adipose Stromal Vascular Fraction Cells Isolated with a Closed System Device in an Immunocompetent Murine Pressure Ulcer Model"],"prefix":"10.1177","volume":"29","author":[{"given":"Joanna","family":"Bukowska","sequence":"first","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Alarcon Uquillas","sequence":"additional","affiliation":[{"name":"LaCell LLC, New Orleans, Louisiana."},{"name":"Obatala Sciences, Inc., New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiying","family":"Wu","sequence":"additional","affiliation":[{"name":"LaCell LLC, New Orleans, Louisiana."},{"name":"Obatala Sciences, Inc., New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trivia","family":"Frazier","sequence":"additional","affiliation":[{"name":"LaCell LLC, New Orleans, Louisiana."},{"name":"Obatala Sciences, Inc., New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Walendzik","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikaela","family":"Vanek","sequence":"additional","affiliation":[{"name":"LaCell LLC, New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina","family":"Gaupp","sequence":"additional","affiliation":[{"name":"Center for Stem Cell Research &amp; Regenerative Medicine, Tulane University School of Medicine, New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce A.","family":"Bunnell","sequence":"additional","affiliation":[{"name":"Center for Stem Cell Research &amp; Regenerative Medicine, Tulane University School of Medicine, New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Kosnik","sequence":"additional","affiliation":[{"name":"Tissue Genesis LLC, Houston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babak","family":"Mehrara","sequence":"additional","affiliation":[{"name":"Memorial Sloan Kettering Cancer Center, New York, New York."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Katz","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Wake Forest School of Medicine, Winston Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Gawronska-Kozak","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Gimble","sequence":"additional","affiliation":[{"name":"LaCell LLC, New Orleans, Louisiana."},{"name":"Obatala Sciences, Inc., New Orleans, Louisiana."},{"name":"Center for Stem Cell Research &amp; Regenerative Medicine, Tulane University School of Medicine, New Orleans, Louisiana."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,4,2]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2011.02.001"},{"key":"e_1_3_4_3_2","first-page":"22","article-title":"Prevalence of pressure ulcers in Canadian healthcare settings","volume":"50","author":"Woodbury MG","year":"2004","unstructured":"Woodbury MG, and Houghton PE. 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