{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:29:15Z","timestamp":1777933755090,"version":"3.51.4"},"reference-count":63,"publisher":"SAGE Publications","issue":"15","license":[{"start":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T00:00:00Z","timestamp":1596067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p>Perivascular areas of the brain harbor multipotent stem cells. We recently demonstrated that after a stroke, brain pericytes exhibit features of multipotent stem cells. Moreover, these ischemia-induced multipotent stem cells (iSCs) are present within ischemic areas of the brain of patients diagnosed with stroke. Although increasing evidence shows that iSCs have traits similar to those of mesenchymal stem cells (MSCs), the phenotypic similarities and differences between iSCs and MSCs remain unclear. In this study, we used iSCs extracted from stroke patients (h-iSCs) and compared their neurogenic potential with that of human MSCs (h-MSCs) in vitro. Microarray analysis, fluorescence-activated cell sorting, immunohistochemistry, and multielectrode array were performed to compare the characteristics of h-iSCs and h-MSCs. Although h-iSCs and h-MSCs had similar gene expression profiles, the percentage expressing the neural stem\/progenitor cell marker nestin was significantly higher in h-iSCs than in h-MSCs. Consistent with these findings, h-iSCs, but not h-MSCs, differentiated into electrophysiologically functional neurons. In contrast, although both h-iSCs and h-MSCs were able to differentiate into several mesodermal lineages, including adipocytes, osteocytes, and chondrocytes, the potential of h-iSCs to differentiate into adipocytes and osteocytes was relatively low. These results suggest that compared with h-MSCs, h-iSCs predominantly exhibit neural rather than mesenchymal lineages. In addition, these results indicate that h-iSCs have the potential to repair the injured brain of patients with stroke by directly differentiating into neuronal lineages.<\/jats:p>","DOI":"10.1089\/scd.2020.0031","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T07:16:48Z","timestamp":1591687008000},"page":"994-1006","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Ischemia-Induced Multipotent Stem Cells Isolated from Stroke Patients Exhibit Higher Neurogenic Differentiation Potential than Bone Marrow-Derived Mesenchymal Stem Cells"],"prefix":"10.1177","volume":"29","author":[{"given":"Saujanya","family":"Rajbhandari","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiya","family":"Beppu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshinori","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Nakano-Doi","sequence":"additional","affiliation":[{"name":"Institute for Advanced Medical Sciences, Departments of Hyogo College of Medicine, Nishinomiya, Japan."},{"name":"Therapeutic Progress in Brain Diseases and Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nami","family":"Nakagomi","sequence":"additional","affiliation":[{"name":"Surgical Pathology, Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Matsuyama","sequence":"additional","affiliation":[{"name":"Therapeutic Progress in Brain Diseases and Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Nakagomi","sequence":"additional","affiliation":[{"name":"Institute for Advanced Medical Sciences, Departments of Hyogo College of Medicine, Nishinomiya, Japan."},{"name":"Therapeutic Progress in Brain Diseases and Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan."},{"name":"Institute for Advanced Medical Sciences, Departments of Hyogo College of Medicine, Nishinomiya, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"e_1_3_5_2_2","first-page":"880","article-title":"Thrombolytic therapy of acute ischemic stroke: correlation of angiographic recanalization with clinical outcome","volume":"26","author":"Zaidat OO","year":"2005","unstructured":"Zaidat OO, , Suarez JI, , Sunshine JL, , Tarr RW, , Alexander MJ, , Smith TP, , Enterline DS, , Selman WR, and Landis DMD. 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