{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:30:11Z","timestamp":1777933811705,"version":"3.51.4"},"reference-count":49,"publisher":"SAGE Publications","issue":"14","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>\n                    Human pluripotent stem cells (hPSCs) have generated significant interest in the scientific community based on their potential applications in regenerative medicine. However, numerous research groups have reported a propensity for genomic alterations during hPSC culture that poses concerns for basic research and clinical applications. Work from our laboratory and others has demonstrated that amplification of chromosomal regions is correlated with increased gene expression. To date, the phenotypic association of common genomic alterations remains unclear and is a cause for concern during clinical use. In this study, we focus on trisomy 17 and a list of candidate genes with increased gene expression to hypothesize that overexpressing 17q25 located\n                    <jats:italic toggle=\"yes\">ARHGDIA<\/jats:italic>\n                    will confer selective advantage to hPSCs. HPSC lines overexpressing\n                    <jats:italic toggle=\"yes\">ARHGDIA<\/jats:italic>\n                    exhibited culture dominance in co-cultures of overexpression lines with nonoverexpression lines. Furthermore, during low-density seeding, we demonstrate increased clonality of our ARHGDIA lines against matched controls. A striking observation is that we could reduce this selective advantage by varying the hPSC culture conditions with the addition of ROCK inhibitor (ROCKi). This work is unique in (1) demonstrating a novel gene that confers selective advantage to hPSCs when overexpressed and may help explain a common trisomy dominance, (2) providing a selection model for studying culture conditions that reduce the appearance of genomically altered hPSCs, and (3) aiding in elucidation of a mechanism that may act as a molecular switch during culture adaptation.\n                  <\/jats:p>","DOI":"10.1089\/scd.2021.0079","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T06:03:03Z","timestamp":1622008983000},"page":"705-713","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>ARHGDIA<\/i>\n                    Confers Selective Advantage to Dissociated Human Pluripotent Stem Cells"],"prefix":"10.1177","volume":"30","author":[{"given":"Marion J.","family":"Riggs","sequence":"first","affiliation":[{"name":"Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, Virginia, USA."},{"name":"Center for Genomic Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA."},{"name":"Department of Neurology, Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"Sheridan","sequence":"additional","affiliation":[{"name":"Center for Quantitative Health, Center for Genomic Medicine and Department of Psychiatry, Massachusetts General Hospital, Boston, Massachusetts, USA."},{"name":"Department of Psychiatry, Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0544-413X","authenticated-orcid":false,"given":"Raj R.","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, Virginia, USA."},{"name":"Department of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, Arkansas, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1145"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.12.003"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0105-19"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2051"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00623"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20897"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.06.018"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002024107"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-201-4_11"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0657"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00011"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.06.017"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1310"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2012.09.001"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401346101"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15271-3"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1285"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22312"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.412"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00256-2"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20245"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07213"},{"key":"e_1_3_4_25_2","first-page":"1182","article-title":"National Institutes of Health","volume":"307","author":"Kaiser J","year":"2005","unstructured":"Kaiser J. 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