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This study aimed to develop a novel method to differentiate bone marrow mesenchymal stem cells (BM-MSCs) into LESC-like cells using a culture medium and paired box 6 (Pax6) transfection. The LESC-like cells were confirmed using the LESC markers CK14 and p63 and CEC marker CK12. Pax6 induces BM-MSCs to differentiate into LESC-like cells in vitro. Mouse models of chemical corneal burn were obtained and treated with the LESC-like cells. The transplantation experiment indicated that Pax6-reprogrammed BM-MSCs attached to and replenished the damaged cornea through the formation of stratified corneal epithelium. The proliferation and colony formation abilities of Pax6-overexpressing BM-MSCs were significantly enhanced. These findings provide evidence that BM-MSCs might serve as an excellent candidate for generating bioengineered corneal epithelium and provide a new strategy for the treatment of clinical corneal damage.<\/jats:p>","DOI":"10.1089\/scd.2022.0249","type":"journal-article","created":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T11:26:50Z","timestamp":1682422010000},"page":"410-421","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Pax6-Induced Proliferation and Differentiation of Bone Marrow Mesenchymal Stem Cells Into Limbal Epithelial Stem Cells"],"prefix":"10.1177","volume":"32","author":[{"given":"Jie","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."},{"name":"Translational Medicine Research Center, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xin","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuandi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keke","family":"He","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6922-6281","authenticated-orcid":false,"given":"Min","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."},{"name":"National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Province Key Laboratory of Regenerative Medicine, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical, Guizhou Medical University, Guiyang, Guizhou, China."},{"name":"Translational Medicine Research Center, Guizhou Medical University, Guiyang, Guizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31814fa814"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12886-022-02326-w"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2020.10.006"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pop.2015.05.004"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb7010005"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.836009"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2016.0388"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40123-020-00305-2"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-013-9451-6"},{"key":"e_1_3_4_11_2","first-page":"1021","article-title":"Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells","volume":"10","author":"Wang HX","year":"2017","unstructured":"Wang HX, , Gao XW, , Ren B, , Cai Y, , Li W-J, , Yang Y-L, and Li Y-J. 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