{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T21:43:59Z","timestamp":1787867039846,"version":"build-2784847793"},"reference-count":20,"publisher":"SAGE Publications","issue":"9-10","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>Replacement teeth develop from the successional dental lamina (SDL). Understanding how SDL transitions from quiescence to initiation is crucial for preserving dental lamina stem cells in the jawbone microenvironment and for complete tooth regeneration. Miniature pigs are good models for studying human tooth replacement because of their similarities to humans. However, the molecular mechanisms and cellular composition that initiate SDL development remain unclear. One possible reason for this is the limitations of the current methods for culturing SDL in vitro, such as the inability to directly observe tooth morphological changes during culture and low tissue viability. This study aimed to improve the in vitro culture method for SDL. Using a McIlwain Tissue Chopper, we obtained mandibular slices containing deciduous canine and SDL of permanent canine. The slices were approximately 500 \u03bcm thick and were cultured on a Transwell membrane supported with metal grids over medium. The SDL developed into the bud stage on the second day and entered the cap stage on the fifth day in vitro. The expression of proliferation markers, cell death markers, and key odontogenetic genes in vitro was similar to that observed in vivo. In conclusion, we successfully applied a slice culture system to the SDL of miniature pigs. This slice culture method allowed us to directly visualize SDL initiation and further elucidate the molecular mechanisms underlying the initiation of permanent tooth development.<\/jats:p>","DOI":"10.1089\/scd.2024.0044","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T09:20:23Z","timestamp":1712222423000},"page":"239-248","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of Slice Culture System for Successional Dental Lamina in Diphyodont Mammals"],"prefix":"10.1177","volume":"33","author":[{"given":"Qiong","family":"Li","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Lin","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."},{"name":"Department of Oral Pathology, Peking University Sc hool and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinsong","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."},{"name":"Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."},{"name":"Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, People\u2019s Republic of China."},{"name":"Academician Workstation for Oral-Maxillofacial Regenerative Medicine, Central South University, Changsha, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7003-0884","authenticated-orcid":false,"given":"Xiaoshan","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and School of Stomatology, Capital Medical University, Beijing, People\u2019s Republic of China."},{"name":"Academician Workstation for Oral-Maxillofacial Regenerative Medicine, Central South University, Changsha, People\u2019s Republic of China."},{"name":"Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.052415"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.171363"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1069>3.0.CO;2-Z"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.09.003"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213202110"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2008.00258.x"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2007.00499.x"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21275"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512442896"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12126"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.03.004"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019102374"},{"key":"e_1_3_5_14_2","first-page":"e50824","article-title":"The slice culture method for following development of tooth germs in explant culture","author":"Alfaqeeh SA","year":"2013","unstructured":"Alfaqeeh SA, , Tucker AS. 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