{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:25:22Z","timestamp":1789622722050,"version":"build-2803163510"},"reference-count":36,"publisher":"SAGE Publications","issue":"17-18","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:p>\n                    Adipose stem cells are considered one of the primary drivers of autologous fat graft biological activity and survival. We have previously demonstrated that hormonally active VD3 improved adipose stem cell viability in ex vivo and in vivo fat grafting models. In this study, we evaluated the inactive form of VD3 (cholecalciferol) on adipose stromal cell (ASC) phenotype during hypoxia and the subsequent effect on human fat graft retention in the xenograft model. Lipoaspirate collected from six human donors was used for ex vivo particle culture studies and isolated ASC studies. Adipose particles were treated with increasing doses of VD3 to determine impact on ASC survival. Expanded stromal cells were treated with VD3 during hypoxic culture and assessed for viability, apoptosis, mitochondrial activity, and nitric oxide (NO) release via caspase, DAF-FM, or TMRM. Finally, 40 Nu\/J mice receiving bilateral dorsal human lipoaspirate were treated thrice weekly with (1) vehicle control, (2) 50 ng calcitriol, (3) 50 ng VD3, (4) 500 ng VD3, and (5) 5,000 ng VD3 for 12 weeks,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 8 per group. Graft weight, volume, and architecture were analyzed. Adipose particles treated with dose-escalating VD3 had significantly increased ASC viability compared with control (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01). Under hypoxia, ASCs treated with 1 nM VD3 had significantly greater viability than untreated and pretreated cells (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01) and significantly lower apoptosis-to-viability ratio (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01). ASCs pretreated with 1 nM VD3 had significantly lower NO release (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.05) and lower mitochondrial polarization (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.05) compared with controls. In vivo results showed mice receiving 5,000 ng VD3 had significantly greater graft weight (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.05) and volume (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.05) after 12 weeks of treatment compared with controls. Grafts had enhanced neovascularization, intact adipocyte architecture, and absence of oil cysts. VD3 is an over-the-counter nutritional supplement with a known safety profile in humans. Our xenograft model suggests administering VD3 at the time of surgery may significantly improve fat graft retention.\n                  <\/jats:p>","DOI":"10.1089\/scd.2024.0056","type":"journal-article","created":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T02:01:35Z","timestamp":1719626495000},"page":"468-476","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Vitamin D3 Improves Adipose Stromal Cell Survival and Human Fat Graft Retention in Xenograft Model"],"prefix":"10.1177","volume":"33","author":[{"given":"Andreea","family":"Gavrilescu","sequence":"first","affiliation":[{"name":"University of Washington School of Medicine, Seattle, Washington, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn J.","family":"Loder","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Ricketts","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Plastic Surgery, University of Utah Health, Salt Lake City, Utah, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Ramkumar","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahaa","family":"Shaaban","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amr","family":"Elmeanawy","sequence":"additional","affiliation":[{"name":"Plastic Surgery Department, Menoufia University, Shebin El Kom, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Vagonis","sequence":"additional","affiliation":[{"name":"Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A.","family":"Gusenoff","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."},{"name":"McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Peter","family":"Rubin","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."},{"name":"McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5582-8871","authenticated-orcid":false,"given":"Lauren E.","family":"Kokai","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA."},{"name":"McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania, USA."},{"name":"Department of Bioengineering, University of Pittsburgh School of Engineering, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0000000000003916"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1962.00740050419005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2021.02.057"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/ans.18231"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(97)90535-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000002667"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00266-011-9835-4"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1473-2130.2004.00060.x"},{"key":"e_1_3_3_10_2","volume-title":"Autologous Fat Transfer: Art, Science, and Clinical Practice","author":"Shiffman MA","year":"2009","unstructured":"Shiffman MA. Autologous Fat Transfer: Art, Science, and Clinical Practice. 1 ed: Springer Science & Business Media; 2009.","edition":"1"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archfaci.2008.518"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/asj\/sjad001"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/12.3.514"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12227"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/tri.13323"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-017-0610-9"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3749"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac052066o"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0382-4"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2018.07.006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2012.112"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-3084(90)90156-L"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19970915)66:4<552::AID-JCB14>3.0.CO;2-D"},{"key":"e_1_3_3_24_2","first-page":"979","article-title":"Novel 19-nor-hexafluoride vitamin D3 analog (Ro 25-6760) inhibits human colon cancer in vitro via apoptosis","volume":"14","author":"Evans SR","year":"1999","unstructured":"Evans SR, , Soldatenkov V, , Shchepotin EB, et al. Novel 19-nor-hexafluoride vitamin D3 analog (Ro 25-6760) inhibits human colon cancer in vitro via apoptosis. Int J Oncol, 1999; 14:979\u2013985.","journal-title":"Int J Oncol"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.02046.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11202"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115816"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-014-0778-9"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201558200"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2018.09.001"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00156.2014"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1159\/000330075"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S152822"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000634"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.10.033"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14191"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S358470"}],"container-title":["Stem Cells and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/scd.2024.0056","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/scd.2024.0056","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/scd.2024.0056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T02:01:46Z","timestamp":1777687306000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/scd.2024.0056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":36,"journal-issue":{"issue":"17-18","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1089\/scd.2024.0056"],"URL":"https:\/\/doi.org\/10.1089\/scd.2024.0056","relation":{},"ISSN":["1547-3287","1557-8534"],"issn-type":[{"value":"1547-3287","type":"print"},{"value":"1557-8534","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]}}}