{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:43:01Z","timestamp":1777934581397,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Surgical Infections"],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The sudden outbreak of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in more than 261 million infections and an estimated 5.1 million deaths. Some vital organs such as the kidneys, heart, intestines, and lungs could be damaged by SARS-CoV-2. Questions remain regarding the effect of SARS-CoV-2 on the mucous membrane of the appendix in children. The aim of this study was to evaluate the knowledge of cytologic processes in appendix tissue in children with coronavirus disease 2019 (COVID-19).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods:<\/jats:title>\n                    <jats:p>\n                      Fragments of the appendices of children with confirmed COVID-19 (n\u2009=\u200942) were studied by real-time polymerase chain reaction (PCR) to determine the expression of SARS-CoV-2 RNA and genes encoding protein complexes:\n                      <jats:italic toggle=\"yes\">ACE-2<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Furin<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      We found traces of coronavirus genetic material in the appendices of children with COVID-19. We also found increased expression of\n                      <jats:italic toggle=\"yes\">ACE-2<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Furin<\/jats:italic>\n                      in the tissue, which indicates favorable conditions for coronavirus infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      Viral load in the appendices of children can be reported based on the results of real-time PCR for SARS-CoV-2 and the expression of\n                      <jats:italic toggle=\"yes\">ACE-2<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Furin<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/sur.2021.311","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T09:46:10Z","timestamp":1653471970000},"page":"470-474","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Angiotensin-Converting Enzyme 2<\/i>\n                    and\n                    <i>Furin<\/i>\n                    Expression in the Appendix of Children with COVID-19"],"prefix":"10.1177","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8784-8415","authenticated-orcid":false,"given":"Grigory","family":"Demyashkin","sequence":"first","affiliation":[{"name":"Department of Pathomorphology, National Medical Research Center of 8Radiology, Obninsk, Russia."},{"name":"Institute of Translational Medicine, Sechenov University, Moscow, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8447-2600","authenticated-orcid":false,"given":"Konstantin","family":"Gorokhov","sequence":"additional","affiliation":[{"name":"Department of Pathomorphology, National Medical Research Center of 8Radiology, Obninsk, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3763-7454","authenticated-orcid":false,"given":"Ilya","family":"Zorin","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine named after N.V. Sklifosovsky, Sechenov University, Moscow, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Shchekin","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine named after N.V. Sklifosovsky, Sechenov University, Moscow, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-1164","authenticated-orcid":false,"given":"Petr","family":"Shegay","sequence":"additional","affiliation":[{"name":"Centre of Innovational Radiological and Regenerative Technologies, National Medical Research Center of Radiology, Obninsk, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1344-3292","authenticated-orcid":false,"given":"Andrei","family":"Kaprin","sequence":"additional","affiliation":[{"name":"Department of Oncology, Academician of the Russian Academy of Sciences, Moscow, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/307315"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.19048\/2411-8729-2020-6-2-8-30"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0912-6"},{"key":"e_1_3_5_5_2","first-page":"1843","article-title":"Detection of SARS-CoV-2 in different types of clinical specimens","volume":"323","author":"Wang W","year":"2020","unstructured":"Wang W, , Xu Y, , Gao R, et al. Detection of SARS-CoV-2 in different types of clinical specimens. JAMA, 2020; 323:1843\u20131844.","journal-title":"JAMA"},{"key":"e_1_3_5_6_2","author":"Jaijakul S.","unstructured":"Jaijakul S. Colitis as a sole presentation of SARS-CoV-2 infection: Case report. SN Compr Clin Med [Epub ahead of print; DOI: 10.1007\/s42399-020-00346-5].","journal-title":"SN Compr Clin Med"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000000667"},{"key":"e_1_3_5_8_2","first-page":"e8677","article-title":"Complicated appendicitis in a pediatric patient with COVID-19: A case report","volume":"12","author":"Alsuwailem AB","year":"2020","unstructured":"Alsuwailem AB, , Turkistani R, , Alomari M. Complicated appendicitis in a pediatric patient with COVID-19: A case report. Cureus, 2020; 12:e8677.","journal-title":"Cureus"},{"key":"e_1_3_5_9_2","first-page":"e9394","article-title":"Pseudo-appendicitis in an adolescent with COVID-19","volume":"12","author":"Suwanwongse K","year":"2020","unstructured":"Suwanwongse K, , Shabarek N. Pseudo-appendicitis in an adolescent with COVID-19. Cureus, 2020; 12:e9394.","journal-title":"Cureus"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2021.101651"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2010.07.012"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.9738\/INTSURG-D-13-00098.1"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.052"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-020-09915-3"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1267"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12050491"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-0184-0"}],"container-title":["Surgical Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/sur.2021.311","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/sur.2021.311","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/sur.2021.311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T02:28:19Z","timestamp":1777688899000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/sur.2021.311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,31]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,6,1]]}},"alternative-id":["10.1089\/sur.2021.311"],"URL":"https:\/\/doi.org\/10.1089\/sur.2021.311","relation":{},"ISSN":["1096-2964","1557-8674"],"issn-type":[{"value":"1096-2964","type":"print"},{"value":"1557-8674","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,31]]}}}