{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:24:13Z","timestamp":1787311453842,"version":"build-2736575974"},"reference-count":30,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Surgical Infections"],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>The present work aimed to examine impact of tanshinone IIA on intestinal barrier in sepsis and to explore the underpinning mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Sepsis induction in Sprague-Dawley (SD) rats was conducted via cecal ligation and puncture (CLP), with subsequent intraperitoneal injection of tanshinone IIA. Intestinal permeability was examined 12 h post-operation using the fluorescein isothiocyanate dextran method. Blood and distal ileum tissue samples were collected for Enzyme-Linked Immunosorbent Assay (ELISA) analysis of oxidative stress and inflammatory markers. Histopathologic examination was performed using hematoxylin and eosin staining and the Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) assay. Immunofluorescence and immunoblot were performed for protein detection. In vitro, Caco-2 cells were administered lipopolysaccharide (LPS) followed by tanshinone IIA treatment, and pregnane X receptor (PXR) and cytochrome P450-3A4 (CYP3A4) protein levels were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In sepsis model rats, tanshinone IIA dose-dependently reversed the increased intestinal permeability, bacterial shift rate, ileum Chiu\u2019s score, apoptosis level of ileal mucosa, the elevated serum and ileal Malondialdehyde (MDA), Interleukin-1 beta (IL-1\u03b2), and Tumor Necrosis Factor-alpha (TNF-\u03b1) amounts, and the enhanced ileal expression levels of Proto-oncogene c-Fos (\n                      <jats:italic toggle=\"yes\">c-Fos<\/jats:italic>\n                      ) and tryptase proteins. In addition, tanshinone IIA restored the decreased serum and ileal Superoxide Dismutase (SOD) levels and reversed the reduced ileal expression levels of claudin-1, Junctional Adhesion Molecule (JAM), occludin, and ZO-1. In vitro, tanshinone IIA restored PXR and CYP3A4 levels following LPS stimulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Tanshinone IIA exerts a protective effect in murine CLP-induced sepsis. The underlying mechanism may involve activation of the PXR-CYP3A4 pathway in murine intestinal epithelial cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/sur.2025.012","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:25:25Z","timestamp":1745414725000},"page":"591-599","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Tanshinone IIA Improves Intestinal Barrier Integrity in Septic Rats"],"prefix":"10.1177","volume":"26","author":[{"given":"Lvzhao","family":"Liao","sequence":"first","affiliation":[{"name":"EICU, Zhejiang Hospital, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Xiao","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Xing","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wang","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Zhou","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Li","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sixu","family":"Pan","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nafei","family":"Xu","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronglin","family":"Jiang","sequence":"additional","affiliation":[{"name":"ICU, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,4,23]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e318232db65"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000565"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2013.0020"},{"key":"e_1_3_6_5_2","first-page":"104","article-title":"The research progress of tight junction proteins and intestinal mucosal barrier injury","volume":"21","author":"Ma J","year":"2015","unstructured":"Ma J, , Yu X, , Sun N. 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