{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:44:23Z","timestamp":1777938263906,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T00:00:00Z","timestamp":1274832000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p>\n                    Mesenchymal stem cell (MSC) differentiation by growth factors may be improper due to possibility of clinical risk. We have previously developed a growth-factor-free coculture method and observed rat MSCs differentiated into hepatic progenitor cells. This study was aimed to validate hepatic differentiation potential\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . MSCs from bone marrow of green fluorescent protein\u2013transgenic Sprague-Dawley rats were cocultured with hepatocytes from normal Sprague-Dawley rats, sharing growth-factor-free media. After 14 days, cells were implanted into the spleen of carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2013injured rats and kept for 4 weeks. Fibrosis remarkably decreased in CCl\n                    <jats:sub>4<\/jats:sub>\n                    \/cocultured MSC at weeks 1, 3, and 4. Immunohistochemistry revealed that albumin, \u03b1-fetoprotein, and cytokeratin 19 (CK19) expression was high in CCl\n                    <jats:sub>4<\/jats:sub>\n                    \/cocultured MSC only at week 1. Reverse transcription\u2013polymerase chain reaction and Western blot revealed that CCl\n                    <jats:sub>4<\/jats:sub>\n                    \/cocultured MSC had reduced \u03b1-fetoprotein expression at week 4, whereas\n                    <jats:italic toggle=\"yes\">CK18<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">CK19<\/jats:italic>\n                    exhibited stronger expression. Albumin in CCl\n                    <jats:sub>4<\/jats:sub>\n                    \/cocultured MSC increased at week 4 only in protein level. We assume that cocultured MSCs had stayed at hepatic progenitor stage until week 3, and differentiated into hepatocytes or bile-ductal epithelial cells afterward. Hepatic progenitor cells from MSC differentiation in the growth-factor-free coculture system may contribute to the therapeutic effect for liver disease\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2009.0814","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T08:00:12Z","timestamp":1270108812000},"page":"2649-2659","source":"Crossref","is-referenced-by-count":20,"title":["Therapeutic Potential of Bone-Marrow-Derived Mesenchymal Stem Cells Differentiated with Growth-Factor-Free Coculture Method in Liver-Injured Rats"],"prefix":"10.1177","volume":"16","author":[{"given":"Tian Zhu","family":"Li","sequence":"first","affiliation":[{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Hyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Sun","family":"Lee","sequence":"additional","affiliation":[{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Sik","family":"Kim","sequence":"additional","affiliation":[{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Yonsei University College of Medicine"},{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwal","family":"Suh","sequence":"additional","affiliation":[{"name":"Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,5,26]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006657"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63315-9"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.11.4857"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35070587"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.3.221"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5497.1775"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00328-2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215182"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.17.6489"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/342440a0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113334"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5309.60"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0818"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(00)80218-X"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i29.4497"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-008-0080-x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/107632703322066598"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00060.2007"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880181212"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/107632704322791817"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.236"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2006.0562"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2009.04.023"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.3477"},{"key":"e_1_3_2_27_2","first-page":"5775","article-title":"Coexpression of the c-met proto-oncogene and hepatocyte growth factor in human pancreatic cancer","volume":"54","author":"Ebert M.","year":"1994","unstructured":"Ebert M., , Yokoyama M., , Friess H., , B\u00fcchler M.W., , Korc M. 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