{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:55:46Z","timestamp":1791330946914,"version":"4.3.1"},"reference-count":27,"publisher":"SAGE Publications","issue":"21-22","license":[{"start":{"date-parts":[[2011,7,27]],"date-time":"2011-07-27T00:00:00Z","timestamp":1311724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>\n                    Both surgical tympanoplasty and paper patch grafts are frequently procedured to heal tympanic membrane (TM) perforation or chronic otitis media, despite their many disadvantages. In this study, we report a new healing method of TM perforation by using three-dimensional (3D) porous chitosan scaffolds (3D chitosan scaffolds) as an alternative method to surgical treatment or paper patch graft. Various 3D chitosan scaffolds were prepared; and the structural characteristics, mechanical property,\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    biocompatibility, and healing effects of the 3D chitosan scaffolds as an artificial TM in\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    animal studies were investigated. A 3D chitosan scaffold of 5 wt.% chitosan concentration showed good proliferation of TM cells in an\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    study, as well as suitable structural characteristics and mechanical property, as compared with either 1% or 3% chitosan. In\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    animal studies, 3D chitosan scaffold were able to migrate through the pores and surfaces of TM cells, thus leading to more effective TM regeneration than paper patch technique. Histological observations demonstrated that the regenerated TM with the 3D chitosan scaffold consisted of three (epidermal, connective tissue, and mucosal) layers and were thicker than normal TMs. The 3D chitosan scaffold technique may be an optimal healing method used in lieu of surgical tympanoplasty in certain cases to heal perforated TMs.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2010.0533","type":"journal-article","created":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T06:18:39Z","timestamp":1308637119000},"page":"2763-2772","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":43,"title":["A Healing Method of Tympanic Membrane Perforations Using Three-Dimensional Porous Chitosan Scaffolds"],"prefix":"10.1177","volume":"17","author":[{"given":"Jangho","family":"Kim","sequence":"first","affiliation":[{"name":"Seoul National University"},{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Ajou University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Jun","family":"Choi","sequence":"additional","affiliation":[{"name":"Konyang University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Taek","family":"Lim","sequence":"additional","affiliation":[{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Bin","family":"Lee","sequence":"additional","affiliation":[{"name":"Konyang University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoon","family":"Seonwoo","sequence":"additional","affiliation":[{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pill-Hoon","family":"Choung","sequence":"additional","affiliation":[{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keehyun","family":"Park","sequence":"additional","affiliation":[{"name":"Ajou University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong-Su","family":"Cho","sequence":"additional","affiliation":[{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Hoon","family":"Choung","sequence":"additional","affiliation":[{"name":"Ajou University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Hoon","family":"Chung","sequence":"additional","affiliation":[{"name":"Seoul National University"},{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,7,27]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-6665(20)30467-9"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200104000-00025"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0476"},{"key":"e_1_3_3_5_2","first-page":"125","volume-title":"Transactions of the First of Congress of the International Otological Society (abstract)","author":"Blake C.J.","year":"1887","unstructured":"Blake C.J. Transactions of the First of Congress of the International Otological Society (abstract) New York: D. Appleton and Co., 1887; 125."},{"key":"e_1_3_3_6_2","first-page":"437","article-title":"A clinical study of traumatic tympanic perforation","volume":"23","author":"Chun S.H.","year":"1999","unstructured":"Chun S.H., , Lee D.W., , Shin J.K. A clinical study of traumatic tympanic perforation. 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Efficient treatment of chronic tympanic membrane perforations in animal models by chitosan patch scaffolds. 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