{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:46:05Z","timestamp":1777938365732,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"5-6","license":[{"start":{"date-parts":[[2011,10,28]],"date-time":"2011-10-28T00:00:00Z","timestamp":1319760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n                    Cervical dysfunction contributes to a significant number of preterm births and is a common cause of morbidity and mortality in newborn infants. Cervical dysfunction is related to weakened load bearing properties of the collagen-rich cervical stroma. However, the mechanisms responsible for cervical collagen changes during pregnancy are not well defined. It is known that blood flow and oxygen tension significantly increase in reproductive tissues during pregnancy. To examine the effect of oxygen tension, a key mediator of tissue homeostasis, on the formation of cervical-like tissue\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    , we grew primary human cervical cells in both two-dimensional (2D) and three-dimensional (3D) culture systems at 5% and 20% oxygen. Immunofluorescence studies revealed a stable fibroblast phenotype across six passages in all subjects studied (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =5). In 2D culture for 2 weeks, 20% oxygen was associated with significantly increased collagen gene expression (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt;0.01), increased tissue wet weight (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt;0.01), and increased collagen concentration (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    =0.046). 3D cultures could be followed for significantly longer time frames than 2D cultures (12 weeks vs. 2 weeks). In contrast to 2D cultures, 20% oxygen in 3D cultures was associated with decreased collagen concentration (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt;0.01) and unchanged collagen gene expression, which is similar to cervical collagen changes seen during pregnancy. We infer that 3D culture is more relevant for studying cervical collagen changes\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . The data suggest that increased oxygen tension may be related to significant cervical collagen changes seen in pregnancy.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2011.0309","type":"journal-article","created":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T04:42:30Z","timestamp":1316148150000},"page":"499-507","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Oxygen Tension and Formation of Cervical-Like Tissue in Two-Dimensional and Three-Dimensional Culture"],"prefix":"10.1177","volume":"18","author":[{"given":"Michael","family":"House","sequence":"first","affiliation":[{"name":"Tufts Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Daniel","sequence":"additional","affiliation":[{"name":"Tufts University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirigin","family":"Elstad","sequence":"additional","affiliation":[{"name":"Tufts University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Socrate","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Tufts University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,10,28]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"1","article-title":"Births: final data for 2007","volume":"58","author":"Martin J.A.","year":"2010","unstructured":"Martin J.A., , Hamilton B.E., , Sutton P.D., , Ventura S.J., , Mathews T.J., , Kirmeyer S., , Osterman M.J. Births: final data for 2007. Natl Vital Stat Rep, 58:1. 2010.","journal-title":"Natl Vital Stat Rep"},{"key":"e_1_3_3_3_2","first-page":"1","article-title":"Infant mortality statistics from the 2006 period linked birth\/infant death data set","volume":"58","author":"Mathews T.J.","year":"2010","unstructured":"Mathews T.J., , Macdorman M.F. Infant mortality statistics from the 2006 period linked birth\/infant death data set. Natl Vital Stat Rep, 58:1. 2010.","journal-title":"Natl Vital Stat Rep"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60136-1"},{"key":"e_1_3_3_5_2","volume-title":"Preterm Birth: Causes, Consequences, Prevention","author":"Institute of Medicine","year":"2006","unstructured":"Institute of Medicine. Preterm Birth: Causes, Consequences, Prevention. 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