{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:48:24Z","timestamp":1777938504672,"version":"3.51.4"},"reference-count":38,"publisher":"SAGE Publications","issue":"5-6","license":[{"start":{"date-parts":[[2013,12,2]],"date-time":"2013-12-02T00:00:00Z","timestamp":1385942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>\n                    The aim of this study was to examine the effects of human umbilical cord blood-derived CD34-positive endothelial progenitor cells (CD34+ EPCs) on osteoblastic differentiation of cultured human periosteal-derived osteoblasts (POs). CD34+ cells from human umbilical cord blood were sorted to purify more EPCs in characterization. These sorted cells showed CD31, VE-cadherin, and KDR expression as well as CD34 expression and formed typical tubes in Matrigel. These sorted cells were referred to as human cord blood-derived CD34+ EPCs. In\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    bone formation using a miniature pig model, the newly formed bone was clearly examined in defects filled with polydioxanone\/pluronic F127 (PDO\/Pluronic F127) scaffolds containing either human umbilical cord blood-derived CD34+ EPCs and POs or human umbilical vein endothelial cells (HUVEC) and POs; however, the new bone had the greatest density in the defect treated with CD34+ EPCs and POs. Osteoblastic phenotypes of cultured human POs using ALP activity and von Kossa staining were also more clearly found in CD34+ EPC-conditioned medium than CD34-negative (CD34\u2212) cell-conditioned medium, whereas HUVEC-conditioned medium had an intermediate effect. PCR array for common cytokines and growth factors showed that the secretion of interleukin (IL)-1\u03b2 was significantly higher in CD34+ EPCs than in HUVEC, followed by level in CD34\u2212 cells. In addition, IL-1\u03b2 also potently and dose dependently increased ALP activity and mineralization of POs in culture. These results suggest that human umbilical cord blood-derived CD34+ EPCs stimulates osteoblastic differentiation of cultured human POs. The functional role of human umbilical cord blood-derived CD34+ EPCs in increasing the osteogenic phenotypes of cultured human POs may depend on IL-1\u03b2 secreted from human umbilical cord blood-derived CD34+ EPCs.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2013.0329","type":"journal-article","created":{"date-parts":[[2013,10,30]],"date-time":"2013-10-30T04:01:28Z","timestamp":1383105688000},"page":"940-953","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Human Umbilical Cord Blood-Derived CD34-Positive Endothelial Progenitor Cells Stimulate Osteoblastic Differentiation of Cultured Human Periosteal-Derived Osteoblasts"],"prefix":"10.1177","volume":"20","author":[{"given":"Jin-Ho","family":"Lee","sequence":"first","affiliation":[{"name":"Hannam University","place":["South 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