{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:11:41Z","timestamp":1787008301914,"version":"build-2736575974"},"reference-count":58,"publisher":"SAGE Publications","issue":"5-6","license":[{"start":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T00:00:00Z","timestamp":1415318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>\n                    A key goal for successful bone regeneration is to bridge a bone defect using healing procedures that are stable and durable. Adipose-derived stem cells (ASCs) have the potential to differentiate into bone. Meanwhile, platelet-rich plasma (PRP) is an interesting biological means to repair tissue by inducing chemotactic, proliferative, and anabolic cellular responses. This study evaluated bone regeneration using a combination of ASCs and PRP in a rat calvarial defect model. ASCs were isolated from inguinal fat pads of F344 inbred rats, while PRP was prepared from these rats. ASCs were cultured in control medium supplemented with 10% fetal bovine serum or 5% PRP\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . After 1 week, levels of growth factors including insulin-like growth factor-1, transforming growth factor-\u03b21, hepatocyte growth factor, and vascular endothelial growth factor in the culture supernatant were measured by enzyme-linked immunosorbent assays. Moreover, the ASC\/PRP admixture was transplanted into the rat calvarial defect. Microcomputed tomography, histological, and immunohistochemical (osteopontin and osteocalcin) analyses were performed at 4 and 8 weeks after transplantation. The\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    study showed that the levels of growth factors secreted by ASCs were significantly increased by the addition of PRP. Transplantation of the ASC\/PRP admixture had dramatic effects on bone regeneration overtime in comparison with rats that received other transplants. Furthermore, some ASCs directly differentiated into osteogenic cells\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . These findings suggest that the combination of ASCs and PRP has augmentative effects on bone regeneration. The ASC\/PRP admixture may be a promising source for the clinical treatment of cranial defects.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2014.0336","type":"journal-article","created":{"date-parts":[[2014,10,7]],"date-time":"2014-10-07T01:33:59Z","timestamp":1412645639000},"page":"895-905","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":62,"title":["Direct and Indirect Effects of a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma on Bone Regeneration"],"prefix":"10.1177","volume":"21","author":[{"given":"Satoshi","family":"Tajima","sequence":"first","affiliation":[{"name":"Juntendo University School of Medicine","place":["Japan"]},{"name":"Japan Self Defense Force Yokosuka Hospital","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morikuni","family":"Tobita","sequence":"additional","affiliation":[{"name":"Juntendo University School of Medicine","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakan","family":"Orbay","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiko","family":"Hyakusoku","sequence":"additional","affiliation":[{"name":"Nippon Medical School","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Juntendo University School of Medicine","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,11,7]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4107"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0012"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197905000-00039"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200400026"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb03079.x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-5898(20)30395-3"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1597\/1545-1569_2000_037_0229_moacbg_2.3.co_2"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-9-66"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.2002.130412.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8493529"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5356.1528"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2004.01038.x"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20624"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001886"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000124062.31102.57"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0325"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2007.12.001"},{"key":"e_1_3_4_21_2","first-page":"363","article-title":"A novel approach to periodontal tissue regeneration with mesenchymal stem cells and platelet-rich plasma using tissue engineering technology: a clinical case report","volume":"26","author":"Yamada Y.","year":"2006","unstructured":"Yamada Y., , Ueda M., , Hibi H., , and Baba S. 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