{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:00:31Z","timestamp":1790154031762,"version":"4.0.1"},"reference-count":45,"publisher":"SAGE Publications","issue":"19-20","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:p>\n                    Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea, as well as other tubular organs. A Neo-Urinary Conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed. In this study, we report our findings on the feasibility of using a decellularized trachea for NUC applications. As a NUC scaffold, decellularized trachea provides benefits of having not only naturally occurring biological components but also having sufficient mechanical properties and structural integrity. We, therefore, decellularized rabbit trachea, evaluated its mechanical performance, and investigated its ability to support\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    growth of human smooth muscle cells (hSMCs) and human urothelial cells (hUCs). The decellularized trachea had appropriate biomechanical properties with ultimate tensile strength of \u223c0.34\u2009MPa in longitudinal direction and \u223c1.0\u2009MPa in circumferential direction and resisted a radial burst pressure of &gt;155\u2009mm Hg. Cell morphology study by scanning electron microscopy further showed that hUCs grown on decellularized trachea adopted a typical flatten and interconnected network structure in the lumen of the scaffold, while they formed a round spherical shape and did not spread on the outer surfaces. SMCs, on the other hand, spread well throughout the scaffold. The gene expression analysis by real time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence studies further confirmed scaffold's ability to support long-term growth of hSMCs. Since uroepithelium has been shown to regenerate itself over time\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    , these findings suggest that it is possible to construct a NUC from decellularized trachea without any preseeding of UCs. In future, we plan to translate decellularized trachea in a preclinical animal model and evaluate its biological performance.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2017.0436","type":"journal-article","created":{"date-parts":[[2018,4,13]],"date-time":"2018-04-13T04:27:43Z","timestamp":1523593663000},"page":"1456-1467","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea"],"prefix":"10.1177","volume":"24","author":[{"given":"Anirudha","family":"Singh","sequence":"first","affiliation":[{"name":"The James Buchanan Brady Urological Institute"},{"name":"Johns Hopkins University"},{"name":"Johns Hopkins University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lee","sequence":"additional","affiliation":[{"name":"Johns Hopkins University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harrison","family":"Jeong","sequence":"additional","affiliation":[{"name":"Johns Hopkins University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Yu","sequence":"additional","affiliation":[{"name":"Johns Hopkins University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuru","family":"Li","sequence":"additional","affiliation":[{"name":"Johns Hopkins University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen Hao","family":"Fang","sequence":"additional","affiliation":[{"name":"Johns Hopkins University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveena","family":"Sabnekar","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopu","family":"Liu","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Yoshida","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai","family":"Sopko","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trinity J.","family":"Bivalacqua","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute"},{"name":"Johns Hopkins Medical Institutions and Sidney Kimmel Comprehensive Cancer Center (SKCC)"},{"name":"Johns Hopkins Medical Institutions and Sidney Kimmel Comprehensive Cancer Center 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