{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:05:56Z","timestamp":1791821156984,"version":"4.3.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"3-4","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:p>\n                    In knee osteoarthritis (OA), there is more pronounced cartilage damage in the medial compartment (\u201clesion zone\u201d) than the lateral compartment (\u201cremote zone\u201d). This study fills a gap in the literature by conducting a systematic comparison of cartilage and chondrocyte characteristics from these two zones. It also investigates whether chondrocytes from the different zones respond distinctly to changes in the physical and mechanical microenvironment using three-dimensional porous scaffolds by changing stiffness and pore size. Cartilage was harvested from patients with end-stage varus knee OA. Cartilage from the lesion and remote zones were compared through histological and biomechanical assessments, and through proteomic and gene transcription analyses of chondrocytes. Gelatin scaffolds with varied pore sizes and stiffness were used to investigate\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    microenvironmental regulation of chondrocytes from the two zones. Cartilage from the lesion and remote zones differed significantly (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05) in histological and biomechanical characteristics, as well as phenotype, protein, and gene expression of chondrocytes. Chondrocytes from both zones were sensitive to changes in the structural and mechanical properties of gelatin scaffolds. Of interest, although all chondrocytes better retained chondrocyte phenotype in stiffer scaffolds, those from the lesion and remote zones, respectively, preferred scaffolds with larger and smaller pores. Distinct variations exist in cartilage and chondrocyte characteristics in the lesion and remote zones of knee OA. Cells in these two zones respond differently to variations in the physical and mechanical microenvironment. Understanding and manipulating these differences will facilitate the development of more efficient and precise diagnostic and therapeutic approaches for knee OA.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2020.0085","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T05:20:56Z","timestamp":1592284856000},"page":"223-236","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of Chondrocytes in Knee Osteoarthritis and Regulation by Scaffold Pore Size and Stiffness"],"prefix":"10.1177","volume":"27","author":[{"given":"Yu","family":"Zhao","sequence":"first","affiliation":[{"name":"Arthritis Clinic and Research Center, Peking University People's Hospital, Peking University, Beijing, China."},{"name":"Arthritis Institute, Peking University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"You","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Xing","sequence":"additional","affiliation":[{"name":"Arthritis Clinic and Research Center, Peking University People's Hospital, Peking University, Beijing, China."},{"name":"Arthritis Institute, Peking University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"Kolling Institute, Faculty of Medicine and Health, University of Sydney, St. Leonards, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Arthritis Clinic and Research Center, Peking University People's Hospital, Peking University, Beijing, China."},{"name":"Arthritis Institute, Peking University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesuyuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Arthritis Clinic and Research Center, Peking University People's Hospital, Peking University, Beijing, China."},{"name":"Arthritis Institute, Peking University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikun","family":"Li","sequence":"additional","affiliation":[{"name":"Arthritis Clinic and Research Center, Peking University People's Hospital, Peking University, Beijing, China."},{"name":"Arthritis Institute, Peking University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering and Materials science, Washington University in St. Louis, St. Louis, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaozhao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing, 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