{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T21:38:17Z","timestamp":1784842697446,"version":"3.55.0"},"reference-count":55,"publisher":"SAGE Publications","issue":"17-18","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:p>\n                    Ischemic stroke is a devastating medical condition with poor prognosis due to the lack of effective treatment modalities. Transplantation of human neural stem cells or primary neural cells is a promising treatment approach, but this is hindered by limited suitable cell sources and low\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    expansion capacity. This study aimed (1) use small molecules (SM) to reprogram gingival mesenchymal stem cells (GMSCs) commitment to the neural lineage cells\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    , and (2) use hyaluronic acid (HA) hydrogel scaffolds seeded with GMSCs-derived neural lineage cells to treat ischemic stroke\n                    <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                    . Neural induction was carried out with a SM cocktail-based one-step culture protocol over a period of 24 h. The induced cells were analyzed for expression of neural markers with immunocytochemistry and quantitative real-time polymerase chain reaction (qRT-PCR). The Sprague-Dawley (SD) rats (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 100) were subjected to the middle cerebral artery occlusion (MCAO) reperfusion ischemic stroke model. Then, after 8 days post-MCAO, the modeled rats were randomly assigned to six study groups (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 12 per group): (1) GMSCs, (2) GMSCs-derived neural lineage cells, (3) HA and GMSCs-derived neural lineage cells, (4) HA, (5) PBS, and (6) sham transplantation control, and received their respective transplantation. Evaluation of post-stroke recovery were performed by behavioral tests and histological assessments. The morphologically altered nature of neural lineages has been observed of the GMSCs treated with SMs compared to the untreated controls. As shown by the qRT-PCR and immunocytochemistry, SMs further significantly enhanced the expression level of neural markers of GMSCs as compared with the untreated controls (all\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05). Intracerebral injection of self-assembling HA hydrogel carrying GMSCs-derived neural lineage cells promoted the recovery of neural function and reduced ischemic damage in rats with ischemic stroke, as demonstrated by histological examination and behavioral assessments (all\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05). In conclusion, the SM cocktail significantly enhanced the differentiation of GMSCs into neural lineage cells. The HA hydrogel was found to facilitate the proliferation and differentiation of GMSCs-derived neural lineage cells. Furthermore, HA hydrogel seeded with GMSCs-derived neural lineage cells could promote tissue repair and functional recovery in rats with ischemic stroke and may be a promising alternative treatment modality for stroke.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2023.0330","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T03:21:19Z","timestamp":1715916079000},"page":"563-576","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["An Injectable Hydrogel Loaded with GMSCs-Derived Neural Lineage Cells Promotes Recovery after Stroke"],"prefix":"10.1177","volume":"30","author":[{"given":"Shan","family":"Jiang","sequence":"first","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."},{"name":"Shenzhen Stomatology Hospital (Pingshan), Southern Medical University, Shenzhen, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyong","family":"Yuan","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zou","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."},{"name":"Shenzhen Stomatology Hospital (Pingshan), Southern Medical University, Shenzhen, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Hao","family":"Koh","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Basabrain","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixin","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boon Chin","family":"Heng","sequence":"additional","affiliation":[{"name":"Peking University School of Stomatology, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Wei","family":"Lim","sequence":"additional","affiliation":[{"name":"Li Ka Shing Faculty of Medicine, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penglai","family":"Wang","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengfei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Restorative Dental Sciences, The University of Hong Kong, Hong Kong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1179\/1743132815Y.0000000050"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6223"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4617983"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2329"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1088\/1741-2552\/aa596b"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515604612"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512461016"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902318"},{"key":"e_1_3_5_10_2","first-page":"b18","article-title":"Exploring scarless healing of oral soft tissues","volume":"77","author":"Larjava H","year":"2011","unstructured":"Larjava H, , Wiebe C, , Gallant-Behm C, et al. 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