{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:58:09Z","timestamp":1781845089222,"version":"3.54.5"},"reference-count":95,"publisher":"SAGE Publications","issue":"13-14","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Endothelial cells (ECs) play a crucial role in maintaining tissue homeostasis and functionality. Depending on their tissue of origin, ECs can be highly heterogeneous regarding their morphology, gene and protein expression, functionality, and signaling pathways. Understanding the interaction between organ-specific ECs and their surrounding tissue is therefore critical when investigating tissue homeostasis, disease development, and progression.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    models often lack organ-specific ECs, potentially limiting the translatability and validity of the obtained results. The goal of this study was to assess the differences between commonly used EC sources in tissue engineering applications, including human umbilical vein ECs (HUVECs), human dermal microvascular ECs (hdmvECs), and human foreskin microvascular ECs (hfmvECs), and organ-specific human pancreatic microvascular ECs (hpmvECs), and test their impact on functionality within an\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    pancreas test system used for diabetes research. Utilizing high-resolution Raman microspectroscopy and Raman imaging in combination with established protein and gene expression analyses and exposure to defined physical signals within microfluidic cultures, we identified that ECs exhibit significant differences in their biochemical composition, relevant protein expression, angiogenic potential, and response to the application of mechanical shear stress. Proof-of-concept results showed that the coculture of isolated human islets of Langerhans with hpmvECs significantly increased the functionality when compared with control islets and islets cocultured with HUVECs. Our study demonstrates that the choice of EC type significantly impacts the experimental results, which needs to be considered when implementing ECs into\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    models.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2024.0176","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T13:20:00Z","timestamp":1729862400000},"page":"988-1004","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Heterogeneity of Endothelial Cells Impacts the Functionality of Human Pancreatic\n                    <i>In Vitro<\/i>\n                    Models"],"prefix":"10.1177","volume":"31","author":[{"given":"Max","family":"Urbanczyk","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, Eberhard Karls University 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T\u00fcbingen, Reutlingen, Germany, Reutlingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Loskill","sequence":"additional","affiliation":[{"name":"NMI Natural and Medical Sciences Institute at the University of T\u00fcbingen, Reutlingen, Germany, Reutlingen, Germany."},{"name":"Institute of Biomedical Engineering, Department for Microphysiological Systems, Faculty of Medicine, Eberhard Karls University T\u00fcbingen, T\u00fcbingen, Germany."},{"name":"3R Center T\u00fcbingen for In Vitro Models and Alternatives to Animal Testing, T\u00fcbingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon L.","family":"Layland","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, Eberhard Karls University T\u00fcbingen, T\u00fcbingen, Germany."},{"name":"Department of Women\u2019s Health T\u00fcbingen, University of 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