{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:55:10Z","timestamp":1777938910283,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"3-4","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:p>\n                    Scaffolds made from cartilage extracellular matrix are promising materials for articular cartilage repair, attributed to their intrinsic bioactivity that may promote chondrogenesis. While several cartilage matrix-based scaffolds have supported chondrogenesis\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and\/or\n                    <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                    , it remains a challenge to balance the biological response (e.g., chondroinductivity) with structural (e.g., robust mechanical performance, &gt;1 MPa in compressive stiffness) and translational (e.g., ease of surgical implantation) considerations. Few studies have evaluated encapsulated cell viability within high-stiffness (&gt;1 MPa) hydrogels. We previously fabricated one formulation of a high-stiffness (&gt;3 MPa) pentenoate-functionalized, solubilized, devitalized cartilage (PSDVC) hydrogel that possessed an injectable, paste-like precursor for easy surgical application. In the current study, the characterization of the PSDVC material was expanded by varying the degree of functionalization (i.e., 0.45\u20131.09 mmol\/g) and amount of crosslinker, dithiothreitol (DTT), to improve the reproducibility of the high compressive moduli and evaluate the viability of encapsulated human bone marrow-derived mesenchymal stem cells (hBMSCs) in high-stiffness cartilage matrix hydrogels. Prior to crosslinking, specific formulations functionalized with 0.80 mmol\/g or less of pentenoate groups retained a paste-like precursor rheology. After crosslinking, these formulations produced hydrogels with greater than 1 MPa compressive stiffness. However, hBMSCs encapsulated in PSDVC hydrogels with lower functionalization (i.e., 0.57 mmol\/g, no crosslinker) had a higher stiffness (i.e., 1.4 MPa) but the lowest viability of encapsulated hBMSCs (i.e., 5%). The middle PSDVC functionalization (i.e., 0.70 mmol\/g) with DTT (i.e., 0.50 mmol thiols\/g) demonstrated high cell viability (77%), high mechanical performance (1.65 MPa, 31% failure strain), and translational features (i.e., paste-like precursor, 1.5 min crosslinking time). For future evaluations of PSDVC hydrogels in cartilage repair, a middle functionalization (i.e., 0.70\u20130.80 mmol\/g) with the addition of a crosslinker (i.e., 0.50 mmol thiols\/g) had a desirable balance of high mechanical performance (i.e., &gt;1 MPa compressive stiffness), high viability, and paste-like precursor for surgical translation.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2024.0331","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T11:46:31Z","timestamp":1736768791000},"page":"152-163","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Improved Mesenchymal Stem Cell Viability in High-Stiffness, Translational Cartilage Matrix Hydrogels"],"prefix":"10.1177","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6864-3753","authenticated-orcid":false,"given":"Emi A.","family":"Kiyotake","sequence":"first","affiliation":[{"name":"C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, Colorado, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Iribagiza","sequence":"additional","affiliation":[{"name":"Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krisha","family":"Pramod","sequence":"additional","affiliation":[{"name":"Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Oklahoma, Norman, Oklahoma, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakob M.","family":"Townsend","sequence":"additional","affiliation":[{"name":"C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, Colorado, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7525-8132","authenticated-orcid":false,"given":"Michael S.","family":"Detamore","sequence":"additional","affiliation":[{"name":"C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, Fort Collins, Colorado, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,2,14]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13278"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2022.100343"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2015.0546"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201801501"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.03.009"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom12020216"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40824-023-00349-y"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.04.019"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-011-4248-0"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10614"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2018.0006"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2023.111471"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom12060846"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2019.01.003"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-015-1547-5"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2023.111592"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.06.016"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32563"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2022.0005"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtla.2024.102242"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3tb20745e"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0461"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2009.0761"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1088\/2515-7639\/abdb39"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.07.035"},{"issue":"1","key":"e_1_3_6_28_2","first-page":"70","article-title":"A review on cell damage, viability, and functionality during 3d bioprinting","volume":"9","author":"Xu HQ","year":"2022","unstructured":"Xu HQ, , Liu JC, , Zhang ZY, et al. A review on cell damage, viability, and functionality during 3d bioprinting. Mil Med Res, 2022; 9(1):70; doi: 10.1186\/s40779-022-00429-5","journal-title":"Mil Med Res"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0728-6"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1039\/D2BM01980A"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/polym8080269"},{"key":"e_1_3_6_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz5913"},{"key":"e_1_3_6_33_2","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/ac8e41"},{"key":"e_1_3_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202302571"},{"key":"e_1_3_6_35_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0080"}],"container-title":["Tissue Engineering Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/ten.tea.2024.0331","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ten.tea.2024.0331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ten.tea.2024.0331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:55:52Z","timestamp":1777697752000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/ten.tea.2024.0331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":34,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1089\/ten.tea.2024.0331"],"URL":"https:\/\/doi.org\/10.1089\/ten.tea.2024.0331","relation":{},"ISSN":["1937-3341","1937-335X"],"issn-type":[{"value":"1937-3341","type":"print"},{"value":"1937-335X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]}}}