{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:27:41Z","timestamp":1792189661731,"version":"4.4.0"},"reference-count":51,"publisher":"SAGE Publications","issue":"23-24","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>\n                    Primary open-angle glaucoma is a prevalent type of degenerative eye disease that results in lifelong blindness, and its critical pathogenic cause is trabecular meshwork (TM) dysfunction or decreased TM cellularity. Considering that TM develops from neural crest cells (NCCs), we investigate the potential of human embryonic stem cell (hESC)-derived NCCs transplantation for TM regeneration. We used a chemically defined method to induce the differentiation of NCCs and injected 1.0 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    hESC-derived NCCs combined with 100 \u03bcmol\/L Y-27632 into the anterior chamber of rabbit. Intraocular pressure (IOP), TM, and corneal changes of rabbits with cell transplantation were examined with TonoPEN AVIA, slit lamp microscope, dual-immunofluorescence staining, and optical coherence tomography. The hESC-derived NCCs underwent homogenous differentiation over the course of 5 days\u2019 induction, which expressed the typical neural crest markers HNK-1, P75, SOX10, and AP-2\u03b1. NOD\/SCID mice received injections of hESC-derived NCCs in the groin or axilla. There was no teratoma formation. When intracamerally injected, hESC-derived NCCs integrated into the TM tissue and expressed mature TM cell markers\n                    <jats:italic toggle=\"yes\">Aqp1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Chi3l1<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Timp3<\/jats:italic>\n                    after 7 days transplantation in rabbit eyes. The IOP and central corneal thickness basically maintained at normal levels within 2 weeks. No significant adverse effects in rabbits with hESC-derived NCC injection were observed after 5 weeks of cell transplantation. Our findings indicate that hESC-derived NCCs could integrate into the TM tissue and differentiate into mature TM cells after being injected intracamerally, showing a potential therapeutic approach to addressing TM dysfunction in the treatment of glaucoma.\n                  <\/jats:p>","DOI":"10.1089\/ten.tea.2024.0343","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T04:49:36Z","timestamp":1750049376000},"page":"1309-1318","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>In Vivo<\/i>\n                    Differentiation of hESC-Derived Neural Crest Cells into Trabecular Meshwork Cells"],"prefix":"10.1177","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7419-2998","authenticated-orcid":false,"given":"Ying","family":"Su","sequence":"first","affiliation":[{"name":"School of Clinical Medicine, Shandong Second Medical University, Weifang, People\u2019s Republic of China."},{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."},{"name":"Clinical Skills Teaching Center, Fujian University of Traditional Chinese Medicine, Fuzhou, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyun","family":"Duan","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology, Qingdao Municipal Hospital Affiliated to Qingdao Medical College of Qingdao University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Dong","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulei","family":"Zou","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianwen","family":"Bu","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy, Qingdao University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongyi","family":"Li","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Pan","sequence":"additional","affiliation":[{"name":"Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Disease, School of Ophthalmology Shandong First Medical University, Qingdao, People\u2019s Republic of 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