{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:59:33Z","timestamp":1777939173697,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2010,3,3]],"date-time":"2010-03-03T00:00:00Z","timestamp":1267574400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:p>\n                    The use of polarized salivary gland cell monolayers has contributed to our understanding of salivary gland physiology. However, these cell models are not representative of glandular epithelium\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    , and, therefore, are not ideal for investigating salivary epithelial functions. The current study has developed a three-dimensional (3D) cell culture model for rat Par-C10 parotid gland cells that forms differentiated acinar-like spheres on Matrigel. These 3D Par-C10 acinar-like spheres display characteristics similar to differentiated acini in salivary glands, including cell polarization, tight junction (TJ) formation required to maintain transepithelial potential difference, basolateral expression of aquaporin-3 and Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \/2Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    cotransporter-1, and responsiveness to the muscarinic receptor agonist carbachol that is decreased by the anion channel blocker diphenylamine-2-carboxylic acid or chloride replacement with gluconate. Incubation of the spheres in the hypertonic medium increased the expression level of the water channel aquaporin-5. Further, the proinflammatory cytokines tumor necrosis factor-\u03b1 and interferon-\u03b3 induced alterations in TJ integrity in the acinar-like spheres without affecting individual cell viability, suggesting that cytokines may affect salivary gland function by altering TJ integrity. Thus, 3D Par-C10 acinar-like spheres represent a novel\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    model to study physiological and pathophysiological functions of differentiated acini.\n                  <\/jats:p>","DOI":"10.1089\/ten.tec.2009.0438","type":"journal-article","created":{"date-parts":[[2010,2,2]],"date-time":"2010-02-02T13:25:45Z","timestamp":1265117145000},"page":"1135-1144","source":"Crossref","is-referenced-by-count":36,"title":["Rat Parotid Gland Cell Differentiation in Three-Dimensional Culture"],"prefix":"10.1177","volume":"16","author":[{"given":"Olga J.","family":"Baker","sequence":"first","affiliation":[{"name":"University at Buffalo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Schulz","sequence":"additional","affiliation":[{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean M.","family":"Camden","sequence":"additional","affiliation":[{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongji","family":"Liao","sequence":"additional","affiliation":[{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Troy S.","family":"Peterson","sequence":"additional","affiliation":[{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheikh I.","family":"Seye","sequence":"additional","affiliation":[{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Petris","sequence":"additional","affiliation":[{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary A.","family":"Weisman","sequence":"additional","affiliation":[{"name":"University of Missouri"},{"name":"University of Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,3,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.67.041703.084745"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(83)90165-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00363690"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.279.5.G851"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.22.010305.104219"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2008.0044"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00144.2008"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.2.C367"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-998-0054-5"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-006-0157-3"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-006-0158-2"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501301200"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702767"},{"key":"e_1_3_3_15_2","first-page":"97","article-title":"Altered expression of aquaporins in rat submandibular glands after parasympathetic denervation","volume":"7","author":"Jung J.","year":"2003","unstructured":"Jung J., , Kang O., , Kim W. 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