{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:49:29Z","timestamp":1791773369211,"version":"4.3.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2010,11,4]],"date-time":"2010-11-04T00:00:00Z","timestamp":1288828800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2011,2,1]]},"abstract":"<jats:p>Methods for seeding high-viability (&gt;85%) three-dimensional (3D) alginate\u2013chondrocyte hydrogel scaffolds are presented that employ photocrosslinking of methacrylate-modified alginate with the photoinitiator VA-086. Comparison with results from several other photoinitiators, including Irgacure 2959, highlights the role of solvent, ultraviolet exposure, and photoinitiator cytotoxicity on process viability of bovine chondrocytes in two-dimensional culture. The radicals generated from VA-086 photodissociation are shown to be noncytotoxic at w\/v concentrations up to 1.5%, enabling photocrosslinking without significant cell death. The applicability of these photoinitiators for generating 3D tissue-engineered constructs is evaluated by measuring cell viability in 3D constructs with aggregate moduli in the 10\u201320\u2009kPa range. Hydrogels with encapsulated bovine chondrocytes were constructed with &gt;85% viability using VA-086. While the commonly used Irgacure 2959 is noncytotoxic in its native state and crosslinks the alginate at weight fractions much lower than VA-086, the cytotoxicity of IRG2959's photogenerated radical leads to viabilities below 70% in the conditions tested.<\/jats:p>","DOI":"10.1089\/ten.tec.2009.0582","type":"journal-article","created":{"date-parts":[[2010,8,12]],"date-time":"2010-08-12T09:53:38Z","timestamp":1281606818000},"page":"173-179","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":186,"title":["Methods for Photocrosslinking Alginate Hydrogel Scaffolds with High Cell Viability"],"prefix":"10.1177","volume":"17","author":[{"given":"Andrew D.","family":"Rouillard","sequence":"first","affiliation":[{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline M.","family":"Berglund","sequence":"additional","affiliation":[{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Youn","family":"Lee","sequence":"additional","affiliation":[{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Polacheck","sequence":"additional","affiliation":[{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Tsui","sequence":"additional","affiliation":[{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence J.","family":"Bonassar","sequence":"additional","affiliation":[{"name":"Cornell University"},{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Kirby","sequence":"additional","affiliation":[{"name":"Cornell University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,11,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2003.11.023"},{"key":"e_1_3_3_3_2","first-page":"21","article-title":"Comparison of 3T3 fibroblasts growth on alginate and polyasparagine (PAA) scaffolds in mouse model","volume":"38","author":"Drewa T.","year":"2008","unstructured":"Drewa T. et al. 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