{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:12:36Z","timestamp":1787170356443,"version":"build-2736575974"},"reference-count":56,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T00:00:00Z","timestamp":1508716800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2018,1,1]]},"abstract":"<jats:p>\n                    Extracellular matrix (ECM) is an essential component of the tissue microenvironment, actively shaping cellular behavior.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    culture systems are often poor in ECM constituents, thus not allowing for naturally occurring cell\u2013ECM interactions. This study reports on a straightforward and efficient method for the generation of ECM scaffolds from lung tissue and its subsequent\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    application using primary lung cells. Mouse lung tissue was subjected to decellularization with 0.2% sodium dodecyl sulfate, hypotonic solutions, and DNase. Resultant ECM scaffolds were devoid of cells and DNA, whereas lung ECM architecture of alveolar region and blood and airway networks were preserved. Scaffolds were predominantly composed of core ECM and ECM-associated proteins such as collagens I-IV, nephronectin, heparan sulfate proteoglycan core protein, and lysyl oxidase homolog 1, among others. When homogenized and applied as coating substrate, ECM supported the attachment of lung fibroblasts (LFs) in a dose-dependent manner. After ECM characterization and biocompatibility tests, a novel\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    platform for three-dimensional (3D) matrix repopulation that permits live imaging of cell\u2013ECM interactions was established. Using this system, LFs colonized the ECM scaffolds, displaying a close-to-native morphology in intimate interaction with the ECM fibers, and showed nuclear translocation of the mechanosensor yes-associated protein (YAP), when compared with cells cultured in two dimensions. In conclusion, we developed a 3D-like culture system, by combining an efficient decellularization method with a live-imaging culture platform, to replicate\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    native lung cell\u2013ECM crosstalk. This is a valuable system that can be easily applied to other organs for ECM-related drug screening, disease modeling, and basic mechanistic studies.\n                  <\/jats:p>","DOI":"10.1089\/ten.tec.2017.0283","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T10:32:23Z","timestamp":1508754743000},"page":"1-13","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Generation of a Close-to-Native\n                    <i>In Vitro<\/i>\n                    System to Study Lung Cells\u2013Extracellular Matrix Crosstalk"],"prefix":"10.1177","volume":"24","author":[{"given":"Zuzana","family":"Garl\u00edkov\u00e1","sequence":"first","affiliation":[{"name":"Masaryk University","place":["Czech Republic"]},{"name":"FNUSA-ICRC\u2014International Clinical Research Center of St. Anne University Hospital Brno","place":["Czech 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