{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:23:59Z","timestamp":1791516239393,"version":"4.3.4"},"reference-count":60,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:p>\n                    Pericardial scaffolds have a wide spectrum of clinical applications ranging from patches for vascular reconstruction and abdominal wall defects to bioprosthetic heart valves. The current gold standard of tissue preparation involves disinfection and cross-linking using glutaraldehyde. However, glutaraldehyde-associated toxicity as well as rapid calcification and premature graft failure represent the major modes of failure.\n                    <jats:sup>1<\/jats:sup>\n                    Therefore, a variety of alternative strategies for tissue conservation have been pursued. However, none of those strategies has substituted glutaraldehyde as the method of choice yet. Furthermore, safe sterilization procedures that are nondetrimental to the tissue's functionality are scarce. We have developed a novel procedure to stabilize and sterilize (S) acellular pericardial scaffolds combining photo-initiated ultraviolet cross-linking (U) with low-energy electron irradiation (LEEI). This SULEEI procedure avoids the use of glutaraldehyde and utilizes LEEI as effective sterilization method. A bioburden of 5.1\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u2009\u00b1\u20094.6\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    viable bacteria could be successfully inactivated by SULEEI treatment applying a surface dose of 30.6\u2009\u00b1\u20092.8\u2009kGy. By challenging high-density polyethylene foil stacks with &gt;10\n                    <jats:sup>6<\/jats:sup>\n                    <jats:italic toggle=\"yes\">Bacillus pumilus<\/jats:italic>\n                    spores in different depths and modeling the dose distribution within the scaffolds, a maximum sample thickness of 175\u2009\u03bcm was determined for successful sterilization. Moreover, SULEEI treatment appeared nondetrimental to the ultimate tensile strength (17.6\u2009\u00b1\u20098.6\u2009MPa vs. 17.4\u2009\u00b1\u20099.6\u2009MPa) of the scaffolds compared with glutaraldehyde-treated pericardia. Cell number and overall metabolic activity of human endothelial cells were significantly higher on SULEEI-treated pericardia compared with control samples. In contrast, no cell proliferation could be detected on glutaraldehyde-treated pericardia. Thus, the SULEEI procedure may be a promising novel procedure for glutaraldehyde-free tissue preparation for pericardium-based tissue transplants and tissue engineering.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>Impact Statement<\/jats:title>\n                    <jats:p>Pericardium-based tissue transplantation is a lifesaving treatment. Commercial glutaraldehyde-treated pericardial tissue exhibits cytotoxicity, which is associated with the accelerated graft failure. Replacement of glutaraldehyde has been suggested to overcome those drawbacks. In this study, we report a toxin-free method that combines tissue stabilization with a terminal sterilization. Our data indicate that the SULEEI procedure, which is part of an issued patent, may be a promising first step toward glutaraldehyde-free pericardium-based tissue transplants. Thus, our results may contribute to improving cardiovascular treatment strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/ten.tec.2018.0285","type":"journal-article","created":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T09:59:52Z","timestamp":1541757592000},"page":"717-729","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Stabilization and Sterilization of Pericardial Scaffolds by Ultraviolet and Low-Energy Electron Irradiation"],"prefix":"10.1177","volume":"24","author":[{"given":"Simona","family":"Walker","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessy","family":"Sch\u00f6nfelder","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sems-Malte","family":"Tugtekin","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Faculty of Medicine CGC, Technische Universit\u00e4t Dresden, Herzzentrum Dresden, Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Wetzel","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael C.","family":"Hacker","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmaceutical Technology, Faculty of Medicine, Leipzig University, Leipzig, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Schulz-Siegmund","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmaceutical Technology, Faculty of Medicine, Leipzig University, Leipzig, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,12,17]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.5402\/2013\/728791"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1987.66.1.0093"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2009.01.027"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(19)39457-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/S10016-006-9102-3"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00148-4"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14746-0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03190.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00431-9"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2007.11.003"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2004.07.007"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-017-9610-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820180703"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-008-0301-9"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.20018"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10460"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10346"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62227-4"},{"key":"e_1_3_3_20_2","unstructured":"DIN EN ISO 11137: Sterilization of health care products\u2014Radiation\u2014Part 2. 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