{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:43:54Z","timestamp":1790646234298,"version":"4.1.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"<jats:p>Innovative therapies combining gene-corrected stem cells and the production of bioengineered tissues to treat epidermolysis bullosa are emerging. However, quantitative tests to measure the adhesion forces between two highly viscoelastic substrates such as those found in bilayered bioengineered skin are needed and are still lacking. The objective of this study was to develop a mechanical test to measure the dermal\u2013epidermal adhesion strength of our bilayered tissue-engineered skin substitute (TES) produced with the self-assembly method. We developed a peel test, which allows the displacement of both skin layers in a T configuration, based on the ASTM International standard. A MATLAB program was written to process and analyze raw data. The experimental setup was tested by measuring the dermal\u2013epidermal adhesion strength in TESs produced with normal or collagen VII-deficient cells. Our peel testing method allowed us to detect the impact of the absence of collagen VII in the dermal\u2013epidermal adhesion strength of TESs and also to examine the progression of the dermal\u2013epidermal adhesion strength in relation to culture time in normal TES.<\/jats:p>\n                  <jats:sec>\n                    <jats:title>Impact statement<\/jats:title>\n                    <jats:p>This study describes a method for assessing the adhesion strength at the dermal\u2013epidermal junction of individual tissue-engineered skin substitute (TES). An ASTM standardized protocol of peel testing was designed to measure this important mechanical property. Our innovative approach will serve as a quality control in the production, improvement, and application of TESs for the treatment of pathologies affecting the dermal\u2013epidermal adhesion such as epidermolysis bullosa. Data presented contribute to research on the interfaces between biological substrates and provide a reference factor for the characterization of products derived from tissue engineering.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/ten.tec.2019.0268","type":"journal-article","created":{"date-parts":[[2020,2,22]],"date-time":"2020-02-22T02:06:51Z","timestamp":1582337211000},"page":"180-189","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Peel Test to Assess the Adhesion Strength of the Dermal\u2013Epidermal Junction in Tissue-Engineered Skin"],"prefix":"10.1177","volume":"26","author":[{"given":"Alex E.","family":"Larose","sequence":"first","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."},{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Dakiw-Piaceski","sequence":"additional","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin A.","family":"Barbier","sequence":"additional","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."},{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Larouche","sequence":"additional","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Gauvin","sequence":"additional","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."},{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Caruso","sequence":"additional","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."},{"name":"Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Universit\u00e9 Laval Cancer Research Center, Universit\u00e9 Laval, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Pope","sequence":"additional","affiliation":[{"name":"Paediatric Dermatology, Department of Paediatrics, The Hospital for Sick Children, University of Toronto, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Germain","sequence":"additional","affiliation":[{"name":"CHU of Qu\u00e9bec\u2014Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University\/LOEX, Qu\u00e9bec, Canada."},{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,3,18]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s41038-017-0103-y"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2016.12.009"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004911"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2013.07.007"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1462399406000123"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2014.01.903"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2006.045302"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2005.039685"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12491885"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1993.04540010049007"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26898"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v035a06"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24487"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.15588"},{"key":"e_1_3_4_16_2","first-page":"47","article-title":"A completely biological tissue-engineered human blood vessel","volume":"12","author":"L'Heureux N.","year":"1998","unstructured":"L'Heureux N., , Paquet S., , Labbe R., , Germain L., , and Auger F.A. A completely biological tissue-engineered human blood vessel. Faseb J, 12, 47, 1998.","journal-title":"Faseb J"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-999-0081-x"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v036a10"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/biores.2016.0036"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200206150-00010"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.1950.64"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1984.tb06599.x"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000104"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13298"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.5114\/ada.2017.72457"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2003.09.003"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2015.0258"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2016.09.040"},{"key":"e_1_3_4_29_2","volume-title":"Stickiness Science and Technology [In French]","author":"Cognard J.","year":"2000","unstructured":"Cognard J. Stickiness Science and Technology [In French]. Lausanne, Switzerland: Presses Polytechniques et Universitaires Romandes, 2000."},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijadhadh.2007.01.001"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19082174"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9473-1_1"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1115\/1.2898733"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-380-0_27"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2011.07.058"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12519989"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0219165"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.04.010"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/JID.2015.364"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.11.038"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2018.06.008"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13744"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2019.03.007"},{"key":"e_1_3_4_44_2","article-title":"Measurement of skin adhesion in recessive dystrophic epidermolysis bullosa patients","author":"Nazaroff J.","year":"2018","unstructured":"Nazaroff J., , Manoukian M., , Barriga M., , Lane A., , Marinkovich M.P., , and Tang J.Y. Measurement of skin adhesion in recessive dystrophic epidermolysis bullosa patients. J Am Acad Dermatol, 2018. [Epub ahead of print]; DOI: 10.1016\/j.jaad.2018.07.034.","journal-title":"J Am Acad Dermatol"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2012.01482.x"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12708"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.15304"}],"container-title":["Tissue Engineering Part C: Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/ten.tec.2019.0268","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ten.tec.2019.0268","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ten.tec.2019.0268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:17:17Z","timestamp":1777699037000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/ten.tec.2019.0268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,1]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3,1]]}},"alternative-id":["10.1089\/ten.tec.2019.0268"],"URL":"https:\/\/doi.org\/10.1089\/ten.tec.2019.0268","relation":{},"ISSN":["1937-3384","1937-3392"],"issn-type":[{"value":"1937-3384","type":"print"},{"value":"1937-3392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,1]]}}}