{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:56:34Z","timestamp":1789188994470,"version":"build-2803163510"},"reference-count":30,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:p>\n                    Establishing a suitable animal model of growth plate injury is necessary to evaluate the effect of tissue engineering scaffolds on repairing the injured growth plate. However, the currently used animal models have limitations. Therefore in this study, we reported and evaluated a new modeling method termed the longitudinal disruption method, which is to make a longitudinal defect in the region of growth plate. To compare this new method with the traditional transverse disruption method, we constructed the models by both methods, respectively. To observe whether bone bridges were formed, histological sections were analyzed by hematoxylin-eosin (HE) and Masson staining at 3 weeks after modeling. The HE and Masson staining results showed the formation of bone bridges in both groups, implying that the two methods successfully injured the growth plate. However, it was unclear whether the exact injury to growth plate caused by both methods was consistent. Therefore, to evaluate the accuracy and precision of modeling method, the X-ray and micro-computed tomography (CT) were performed immediately after modeling. The percentages of accurate defect position in the longitudinal and transverse modeling groups were 88.89% and 55.56%, respectively. The micro-CT results revealed irregularly shaped defect cross sections in the transverse modeling group, whereas the defects in the longitudinal modeling group had regular shapes. The mean defect areas were 10.06\u2009\u00b1\u20090.86 and 12.30\u2009\u00b1\u20092.13\u2009mm\n                    <jats:sup>2<\/jats:sup>\n                    in the longitudinal and transverse modeling groups, respectively, while the difference between the actual area and the expected area were \u22121.94\u2009\u00b1\u20090.86 and \u22127.70\u2009\u00b1\u20092.13\u2009mm\n                    <jats:sup>2<\/jats:sup>\n                    , respectively, showing the high precision of this new method. Altogether, we successfully demonstrated a new method for establishing a rabbit model of growth plate injury, which provides a simple and rapid modeling process, good modeling effect, high modeling accuracy, and convenient scaffold implantation. The new method provides an effective animal model for tissue engineering research on the repair and regeneration of injured growth plate.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>Impact Statement<\/jats:title>\n                    <jats:p>In recent years, an increasing number of studies have used tissue engineering scaffolds in the repair and regeneration of growth plate. However, the currently used animal models have certain limitations in the study of tissue engineering scaffold for growth plate. In this study, a new method is presented to establish a rabbit model of growth plate injury. This method is characterized by simple and rapid modeling process, good modeling effect, high modeling accuracy, and convenient scaffold implantation, which is suitable for the study of the repair effects of tissue engineering scaffolds. Altogether, this method provides an effective animal model for tissue engineering research on growth plate and facilitates the development of tissue engineering research on the repair and regeneration of injured growth plate.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/ten.tec.2022.0105","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T05:57:48Z","timestamp":1660283868000},"page":"489-497","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["A New Method for Modeling Rabbit Growth Plate Injury for the Study of Tissue Engineering Scaffolds"],"prefix":"10.1177","volume":"28","author":[{"given":"Minjie","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihao","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Qiang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China."},{"name":"Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyi","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjie","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8647-4818","authenticated-orcid":false,"given":"Pengfei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2017.0274"},{"key":"e_1_3_4_3_2","author":"Chung R","year":"2014","unstructured":"Chung R, , Xian CJ. Recent research on the growth plate: Mechanisms for growth plate injury repair and potential cell-based therapies for regeneration. J Mol Endocrinol, 2014;53(1):T45\u2013T61; doi: 10.1530\/JME-14-0062.","journal-title":"J Mol Endocrinol"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01241398-198709000-00003"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01241398-199011000-00002"},{"key":"e_1_3_4_6_2","unstructured":"Gigante C Martinez AIC. Desepiphysiodesis and reconstruction of the distal radial growth plate with an autologous iliac crest cartilage graft: A case report and review of literature. J Orthop Case Rep 2019;10(1):70\u201373; doi: 10.13107\/jocr.2019.v10.i01.1642."},{"key":"e_1_3_4_7_2","article-title":"Enlightenment of growth plate regeneration based on cartilage repair theory: A review","author":"Wang X","year":"2021","unstructured":"Wang X, , Li Z, , Wang C, et al. Enlightenment of growth plate regeneration based on cartilage repair theory: A review. Front Bioeng Biotechnol, 2021;9:654087; doi: 10.3389\/fbioe.2021.654087.","journal-title":"Front Bioeng Biotechnol"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/1753193411413069"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","unstructured":"Page WT Szabo RM. Distraction osteogenesis for correction of distal radius deformity after physeal arrest. J Hand Surg Am 2009;34(4):617\u2013626; doi: 10.1016\/j.jhsa.2009.01.017.","DOI":"10.1016\/j.jhsa.2009.01.017"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/01241398-200209000-00001"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2019.0180"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S249829"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119536"},{"key":"e_1_3_4_14_2","article-title":"Progress in articular cartilage tissue engineering: A review on therapeutic cells and macromolecular scaffolds","author":"Zhao Z","year":"2020","unstructured":"Zhao Z, , Fan C, , Chen F, et al. Progress in articular cartilage tissue engineering: A review on therapeutic cells and macromolecular scaffolds. Macromol Biosci, 2020;20(2):e1900278; doi: 10.1002\/mabi.201900278.","journal-title":"Macromol Biosci"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3791\/55571"},{"key":"e_1_3_4_16_2","article-title":"Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs","author":"Li W","year":"2017","unstructured":"Li W, , Xu R, , Huang J, et al. Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs. Sci Rep, 2017;7:44140; doi: 10.1038\/srep44140.","journal-title":"Sci Rep"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/biores.2014.0034"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/term.1670"},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","unstructured":"Langer R Vacanti JP. Tissue engineering. Science 1993;260(5110):920\u2013926; doi: 10.1126\/science.8493529.","DOI":"10.1126\/science.8493529"},{"key":"e_1_3_4_20_2","article-title":"Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges","author":"Wei W","year":"2021","unstructured":"Wei W, , Dai H. Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges. Bioact Mater, 2021;6(12):4830\u20134855; doi: 10.1016\/j.bioactmat.2021.05.011.","journal-title":"Bioact Mater"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0437"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5b08911"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/5\/4\/045001"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.06.005"},{"key":"e_1_3_4_25_2","article-title":"3D bioprinting photo-crosslinkable hydrogels for bone and cartilage repair","author":"Mei Q","year":"2021","unstructured":"Mei Q, , Rao J, , Bei HP, et al. 3D bioprinting photo-crosslinkable hydrogels for bone and cartilage repair. Int J Bioprint, 2021;7(3):367; doi: 10.18063\/ijb.v7i3.367.","journal-title":"Int J Bioprint"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","unstructured":"Zhang B Huang J Narayan RJ. Gradient scaffolds for osteochondral tissue engineering and regeneration. J Mater Chem B 2020;8(36):8149\u20138170; doi: 10.1039\/d0tb00688b.","DOI":"10.1039\/D0TB00688B"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","unstructured":"Lee MA Nissen TP Otsuka NY. Utilization of a murine model to investigate the molecular process of transphyseal bone formation. J Pediatr Orthop 2000;20(6):802\u2013806; doi: 10.1097\/00004694-200011000-00021.","DOI":"10.1097\/01241398-200011000-00021"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.orthres.2003.08.003"},{"key":"e_1_3_4_29_2","article-title":"Exosome-loaded extracellular matrix-mimic hydrogel with anti-inflammatory property facilitates\/promotes growth plate injury repair","author":"Guan P","year":"2022","unstructured":"Guan P, , Liu C, , Xie D, et al. Exosome-loaded extracellular matrix-mimic hydrogel with anti-inflammatory property facilitates\/promotes growth plate injury repair. Bioact Mater, 2022;10:145\u2013158; doi: 10.1016\/j.bioactmat.2021.09.010.","journal-title":"Bioact Mater"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/0885328221999894"},{"key":"e_1_3_4_31_2","article-title":"Chitin as a scaffold for mesenchymal stem cells transfers in the treatment of partial growth arrest","author":"Li L","year":"2004","unstructured":"Li L, , Hui JH, , Goh JC, et al. Chitin as a scaffold for mesenchymal stem cells transfers in the treatment of partial growth arrest. J Pediatr Orthop, 2004;24(2):205\u2013210; doi: 10.1097\/00004694-200403000-00014.","journal-title":"J Pediatr Orthop"}],"container-title":["Tissue Engineering Part C: Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/ten.tec.2022.0105","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ten.tec.2022.0105","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ten.tec.2022.0105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:19:23Z","timestamp":1777699163000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/ten.tec.2022.0105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,1]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9,1]]}},"alternative-id":["10.1089\/ten.tec.2022.0105"],"URL":"https:\/\/doi.org\/10.1089\/ten.tec.2022.0105","relation":{},"ISSN":["1937-3384","1937-3392"],"issn-type":[{"value":"1937-3384","type":"print"},{"value":"1937-3392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,1]]}}}