{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:25:17Z","timestamp":1784035517864,"version":"3.55.0"},"reference-count":27,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:p>\n                    Isolated individual myofibers are valuable experimental models that can be used in various conditions to understand skeletal muscle physiology and pathophysiology at the tissue and cellular level. This report details a time- and cost-effective method for isolation of single myofibers from the flexor digitorum brevis (FDB) muscle in both young and aged mice. The FDB muscle was chosen for its documented history in single myofiber experiments. By modifying published methods for FDB myofiber isolation, we have optimized the protocol by first separating FDB muscle into individual bundles before the digestion, followed by optimizing the subsequent digestion medium conditions to ensure reproducibility. Morphological and functional assessments demonstrate a high yield of isolated FDB myofibers with sarcolemma integrity achieved in a shorter time frame than previous published procedures. This method could be also adapted to other types of skeletal muscle. Additionally, this highly reproducible method can greatly reduce the number of animals needed to yield adequate numbers of myofibers for experiments. Thus, this advanced method for myofiber isolation has the potential to accelerate research in skeletal muscle physiology and screening potential therapeutics \u201c\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    \u201d for muscle diseases and regeneration.\n                  <\/jats:p>","DOI":"10.1089\/ten.tec.2023.0012","type":"journal-article","created":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T11:21:29Z","timestamp":1682421689000},"page":"349-360","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Advanced Methodology for Rapid Isolation of Single Myofibers from Flexor Digitorum Brevis Muscle"],"prefix":"10.1177","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-1136","authenticated-orcid":false,"given":"Kamal","family":"Awad","sequence":"first","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan","family":"Moore","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Huang","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Gomez","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Brotto","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venu","family":"Varanasi","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Cardozo","sequence":"additional","affiliation":[{"name":"National Center for the Medical Consequences of Spinal Cord Injury, James J. Peters Department of Veterans Affairs Medical Center, New York, New York, USA."},{"name":"Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Weisleder","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zui","family":"Pan","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5684-7177","authenticated-orcid":false,"given":"Jingsong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynda","family":"Bonewald","sequence":"additional","affiliation":[{"name":"Indiana Center for Musculoskeletal Health, Indiana University Medical School, Indianapolis, Indiana, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Brotto","sequence":"additional","affiliation":[{"name":"Bone Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, University of Texas at Arlington, Arlington, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-014-9915-y"},{"key":"e_1_3_7_3_2","doi-asserted-by":"crossref","unstructured":"Gollapudi SK Michael JJ Chandra M. Striated muscle dynamics. In: Reference Module in Biomedical Sciences. Elsevier Amsterdam; 2014; 1\u201317. 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