{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:56:17Z","timestamp":1789188977104,"version":"build-2803163510"},"reference-count":44,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2024,4,1]]},"abstract":"<jats:p>\n                    Considerable research is being undertaken to develop novel biomaterials-based approaches for surgical reconstruction of bone defects. This extends to three-dimensional (3D) printed materials that provide stable, structural, and functional support\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . However, few preclinical models can simulate\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    human biological conditions for clinically relevant testing. In this study we describe a novel ovine model that allows evaluation of\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    osteogenesis via contact with bone and\/or periosteum interfaced with printed polymer bioreactors loaded with biomaterial bone substitutes. The infraspinous scapular region of 14 Dorset cross sheep was exposed. Vascularized periosteum was elevated either attached to the infraspinatus muscle or separately. In both cases, the periosteum was supplied by the periosteal branch of the circumflex scapular vessels. In eight sheep, a 3D printed 4-chambered polyetheretherketone bioreactor was wrapped circumferentially in vascularized periosteum. In 6 sheep, 12 double-sided 3D printed 2-chambered polyetherketone bioreactors were secured to the underlying bone allowing direct contact with the bone on one side and periosteum on the other. Our model enabled simultaneous testing of up to 24 (12 double-sided) 10\u2009\u00d7\u200910\u2009\u00d7\u20095\u2009mm bioreactors per scapula in the flat contact approach or a single 40\u2009\u00d7\u200910\u2009mm four-chambered bioreactor per scapula using the periosteal wrap.\n                    <jats:italic toggle=\"yes\">De novo<\/jats:italic>\n                    bone growth was evaluated using histological and radiological analysis. Of importance, the experimental model was well tolerated by the animals and provides a versatile approach for comparing the osteogenic potential of cambium on the bone surface and elevated with periosteum. Furthermore, the periosteal flaps were sufficiently large for encasing bioreactors containing biomaterial bone substitutes for applications such as segmental mandibular reconstruction.\n                  <\/jats:p>","DOI":"10.1089\/ten.tec.2023.0345","type":"journal-article","created":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T08:44:40Z","timestamp":1708245880000},"page":"159-169","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Novel Sheep Model to Assess Critical-Sized Bone Regeneration with Periosteum for\n                    <i>In Vivo<\/i>\n                    Bioreactors"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0289-731X","authenticated-orcid":false,"given":"Yohaann A.","family":"Ghosh","sequence":"first","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Sydney Medical School, The University of Sydney, Camperdown, Australia."},{"name":"School of Medicine &amp; Dentistry, Griffith University, Gold Coast, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Xin","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Sydney Medical School, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.S. Abdullah","family":"Al Maruf","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Sydney Medical School, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Royal Prince Alfred Institute of Academic Surgery, Sydney Local Health District, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Innes","family":"Wise","sequence":"additional","affiliation":[{"name":"Laboratory Animal Services, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Burrows","sequence":"additional","affiliation":[{"name":"Laboratory Animal Services, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruta","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica Ka-Yan","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Wykes","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Sydney Medical School, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Leinkram","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona","family":"Froggatt","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery, Chris O'Brien Lifehouse, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Will","family":"Lewin","sequence":"additional","affiliation":[{"name":"Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Camperdown, Australia."},{"name":"Sarcoma and Surgical Research Centre, Chris O'Brien Lifehouse, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hedi V.","family":"Kruse","sequence":"additional","affiliation":[{"name":"Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Sarcoma and Surgical Research Centre, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Faculty of Science, School of Physics, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Tomaskovic-Crook","sequence":"additional","affiliation":[{"name":"Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Camperdown, Australia."},{"name":"Faculty of Science, School of Physics, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"McKenzie","sequence":"additional","affiliation":[{"name":"Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Sarcoma and Surgical Research Centre, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Faculty of Science, School of Physics, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Crook","sequence":"additional","affiliation":[{"name":"Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Camperdown, Australia."},{"name":"Sarcoma and Surgical Research Centre, Chris O'Brien Lifehouse, Camperdown, Australia."},{"name":"Faculty of Science, School of Physics, The University of Sydney, Camperdown, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Head &amp; 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